摘要
目的评价血红素加氧酶-1(HO-1)在白藜芦醇(RES)拮抗阿霉素(DOX)导致淋巴瘤裸鼠动脉内皮细胞凋亡中的作用。方法 40只雄性Balb/c裸鼠经皮下接种Raji细胞建立淋巴瘤模型后随机分成四组:对照组(n=10),DOX组(n=10),DOX+RES组(n=10)和DOX+RES+HO-1抑制剂锡原卟啉(ZnPP)组(n=10)。测定颈动脉组织HO-1、Caspase 3活性。免疫组化方法检测颈动脉HO-1,Bcl-2和Bax表达。应用TUNEL方法检测颈动脉组织细胞凋亡。结果 DOX+RES组较DOX组颈动脉组织HO-1表达和酶活性明显升高(P<0.01),Bcl-2表达升高(P<0.01),Bax表达降低(P<0.01),Caspase-3活性降低(P<0.01)。TUNEL结果提示DOX+RES组较DOX组的动脉内皮凋亡细胞数量明显减少(P<0.01)。RES的上述动脉内皮保护作用被ZnPP明显抑制(P<0.05)。结论 HO-1参与RES对于DOX致淋巴瘤裸鼠动脉内皮细胞凋亡的拮抗作用。
Objective To study the role of riO-1 in the protective effect ofresveratrol （RES） against artery endothelial apoptosis induced by doxorubicin （DOX） in lymphoma nude mice. Methods Forty male Balb/C nude mice after Raji cells injection were randomly divided into four groups （n=10 for each group）： the control group, the DOX group, the DOX＋RES gorup; DOX＋RES＋ZnPP group. The activity of HO-1 and Caspase 3 were determined, and the expression of HO-1, Bcl-2 and Bax protein were observed by immunohistochemisty. Furthermore, endothelial apoptosis was evaluated by TUNEL. Results In the DOX＋RES group, the enzymatic activity and HO-1 expression were enhanced compared with those in the DOX group （P〈0.0 I）, with significantly increased expression of Bel-2 （P〈 0.01）, decreased expression of Bax （P〈0.01）, and decreased activity of Caspase-3 （P〈0.01）. TUNEL results revealed that the number of artery endothelial cells with apoptosis was significantly less in the DOX＋RES group than the DOXgroup （P〈0.01）. The protective effects of RES were significantly inhibited by ZnPP in the DOX＋RES＋ZnPP group （P〈0.05）. Conclusion HO-1 plays a role in the protective effect of RES on DOX-induced artery endothelial apopto- sis in lymphoma nude mice.
出处
《海南医学》
CAS
2012年第23期18-21,共4页
Hainan Medical Journal
基金
上海市卫生局青年科研项目资助(编号:2008Y052)
