乳腺癌易感基因1、P53结合蛋白1、DNA损伤检测点介质1在人喉表皮癌细胞中的表达及临床意义

Expression of Breast Cancer Susceptibility Gene l,P53-binding Protein 1,and Mediator of DNA Damage Checkpoint 1 in Human Laryngeal Carcinoma Cells and Its Clinical Significance

目的研究人喉表皮癌细胞系Hep-2中乳腺癌易感基因1(BRCAl)、P53结合蛋白1(53BP1)和DNA损伤检测点介质1(MDC1)的表达及临床意义。方法采用逆转录聚合酶链式反应检测BRCA1、53BP1、MDC1在喉癌细胞系Hep-2中mRNA的表达,同时用免疫印迹法检测蛋白的表达。结果在所检测的人喉癌细胞系Hep-2中BACR1、53BP1、MDC1在基因与蛋白两个水平均有表达。结论 BRCA1、53BP1、MDC1可能在喉癌的发生发展中有一定作用。

Objective To study the expression of breast cancer susceptibility gene l（BRCA1）,P53-binding protein 1（53BP1） and mediator of DNA damage checkpoint 1（MDC1） in human laryngeal carcinoma cell line Hep-2 and its clinical significance.Methods In human laryngeal carcinoma cell line Hep-2,the expression of BRCA1,53BP1 and MDC1 mRNA were detected by RT-PCR,and the BRCA1,53BP1 and MDC1 proteins expression were measured with Western blotting.Results The expression of BRCA1,53BP1 and MDC1 genes and proteins were all observed in human laryngeal carcinoma cell line Hep-2.Conclusion The expression of BRCA1,53BP1 and MDC1 is probably related to human laryngeal tumorigenesis.