Dlx2条件性激活转基因小鼠模型的建立

Establishment of a Dlx2 conditional activation transgenic mouse model

目的:建立Dlx2条件性激活转基因小鼠,为实现Dlx2基因在不同组织特异性激活奠定基础。方法 :构建Dlx2条件性激活真核表达质粒iZEG-Dlx2并线性化,通过显微注射,获得Dlx2条件性过表达转基因小鼠,应用PCR鉴定其基因型。结果:共获得8只转基因阳性首建鼠,阳性率为11.59%。结论:运用转基因技术,可成功获得Dlx2条件性过表达转基因小鼠,为在体内研究Dlx2基因的功能奠定基础。

PURPOSE： To establish a Dlx2 conditional activation transgenic mouse model, and provide a basis for Dlx2 specific expression in vivo. METHODS： A Dlx2 conditional activation transgenic vector was constructed and named as iZEG-Dlx2.After linearization of the plasmid, pronuclear microinjection technique was applied to introduce the purified DNA into the chromosomes of fertilized mice eggs to obtain transgenic positive founder mouse, and the founder mouse was genotyped by PCR. RESULTS： The Dlx2 conditional transgenic plasmid was constructed successfully and 8 transgenic positive founder mice were obtained, the positive rate was 11.59%. CONCLUSION： Establishment of a Dlx2 conditional activation mouse model by transgenic method was feasible, which may provide basis for the experimental research of Dlx2 molecular function in vivo. Supported by Research Fund of Science and Technology Commission of Shanghai Municipality （10JC1408700）, Shanghai Leading Academic Discipline Project （S30206） and Program for Innovation Research Team of Shanghai Municipal Education Commission.