人类大鼠肉瘤蛋白同源蛋白和高迁移率族蛋白A1在垂体腺瘤中的表达与侵袭性的关系

Relationship between the expression of HRAS and HMGA1 and tumor invasion in human pituitary adenomas

目的研究人类大鼠肉瘤蛋白同源蛋白(V-Ha-Ras harvey rat sarcoma viral oncogene homolog,HRAS)和高迁移率族蛋白A1(High-mobility group A1,HMGA1)在侵袭性和非侵袭性垂体腺瘤组织中的表达情况及二者与垂体腺瘤侵袭性的关系。方法60例垂体腺瘤手术标本按侵袭性垂体腺瘤综合判定方法分为侵袭组和非侵袭组,采用RT-PCR法检测HRAS和HMGA1的mRNA表达水平,采用免疫组织化学法及western blot法检测HRAS和HMGA1的蛋白表达水平,分析侵袭组和非侵袭组HRAS和HMGA1表达水平的差异。结果60例垂体瘤患者中,28例为侵袭性垂体腺瘤,32例为非侵袭性垂体腺瘤。侵袭性垂体腺瘤组HRAS阳性表达率(22/28)、免疫印迹灰度值(1.25±0.16)、mRNA表达量(0.96±0.16)均高于非侵袭性腺瘤组(15/32、0.76±0.10、0.54±0.15)(P<0.05),侵袭性垂体腺瘤组HMGA1阳性表达率(20/28)、免疫印迹灰度值(0.98±0.12)、mRNA表达量(1.12±0.20)均高于非侵袭性腺瘤组(14/32、0.66±0.09、0.52±0.19)(P<0.05),差别具有统计学意义。结论HRAS和HMGA1在侵袭性垂体腺瘤中表达上调,提示二者与垂体腺瘤侵袭性行为的发生有关。

Objective To investigate the expression levels of V-Ha-Ras Harvey Rat Sarcoma Viral Oncogene Homolog（HRAS） and High-mobility group A1 （HMGA1） in the pituitary adenomas and their relationships with tumor invasion in human pituitary adenomas. Methods Sixty specimens of pituitary adenomas were classified into invasive and non-invasive groups according to the comprehensive evaluation of invasive pituitary adenoma. The protein and mRNA expression levels of HRAS and HMGA1 were detected using immunohistoehemistry assay, western blot assay and reverse transcription polymerase chain reaction （RT-PCR） assay, respectively. Results There were twenty-eight cases of invasive pituitary adenomas and 32 eases of non-invasive ones in total 60 specimens. The protein and mRNA expression levels of HRAS were significantly higher in invasive group than in non-invasive group （P 〈 0.05）. The protein and mRNA expression levels of HMGAI were significantly higher in invasive group than in non-invasive group （P 〈 0.05）. Conclusion The present findings suggest that up-regulation of HRAS and HMGA1 can contribute to tu- mor invesion in human pituitary adenomas.