细胞治疗策略促进牵张成骨的研究进展

CELL-BASED APPROACHES TO PROMOTE BONE REGENERATION IN DISTRACTION OSTEOGENESIS

目的综述细胞治疗策略在促进牵张成骨(distraction osteogenesis,DO)方面的研究进展。方法广泛查阅近年来国内外关于细胞治疗策略促进DO、缩短治疗周期的研究文献,对常用方法进行分析总结。结果常用的促进骨再生的细胞治疗策略有细胞注射治疗、细胞-外源性载体支架复合物/可注射组织工程骨、微组织或多细胞聚集体技术、细胞基因治疗,各有优缺点。除自体BMSCs与富血小板血浆复合注射的方法有临床应用报道外,其他方法仍处于基础研究阶段。结论细胞治疗策略在骨再生、促进DO及缩短其治疗周期等方面具有广阔前景,应用于临床前需严谨设计临床前试验来评价其安全性并规范应用方法。

Objective To summarize the recent progress of cell-based approaches tor promoting bone regeneration in distraction osteogenesis （DO）. Methods Recent literature concerning enhancement of bone regeneration following DO using cell-based approaches was reviewed and analyzed. Results An overview of 4 different cell-based approaches was mainly provided： single cell injection, cell scaffold-based strategies/injectable tissue engineered bone, microtissue technology or cell aggregate technology, and stem cell gene therapy. Each has its advantages and disadvantages. Other methods are still in the experimental research except that compound injection of bone marrow mesechymal stem cells and platelet-rich plasma has been applied to clinical practice. Conclusion The cell-based approach is a promising strategy in the field of bone regenerative medicine. These approaches have bright future in promoting bone regeneration and reducing the treatment period in DO in the clinical application. However, well-designed preclinical studies based approaches to promoting bone regeneration during DO. are required to establish safe and effective guidelines for cell-