莪术油对高糖下视网膜血管内皮细胞增殖及VEGF表达的影响

Effects of Rhizoma Zedoariae on proliferation and expression of VEGF of human retinal capillary endothelial cells in high glucose environment

目的:研究不同浓度的莪术油对高糖条件下体外培养的人视网膜血管内皮细胞(humanretinalcapillaryendothelialcells,HRCECs)增殖、调亡及血管内皮细胞生长因子(vascularendothelialgrowthfactor,VEGF)表达的影响。方法:体外培养从角膜移植术后新鲜人眼球提取的HRCECs。取生长良好的第3～4代细胞用于实验,实验分为低糖对照组、高糖对照组、高糖+(60,80,100,120μg/mL)不同浓度莪术油组,用噻唑蓝(MTT)比色法检测HRCECs的增殖,通过免疫细胞化学法观察各分组HRCECs中VEGF的表达情况。结果:MTT比色法结果显示:高糖对照组与低糖对照组没有显著差异(P>0.05),用60,80,100,120μg/mL的莪术油处理高糖下HRCECs作用24h高糖+不同浓度莪术油组与高糖对照组相比具有显著性差异(P<0.05),高糖+不同浓度莪术油组之间呈时间和浓度的显著性差异(P<0.05)。免疫细胞化学检测显示:与低糖对照组相比,高糖对照组VEGF表达明显(P<0.05),用80,100,120μg/mL的莪术油处理高糖下HRCECs24h,高糖+不同浓度莪术油组与高糖对照组相比VEGF表达有显著性差异(P<0.05),高糖+不同浓度莪术油组之间两两比较均具有显著性差异(P<0.05)。结论:莪术油可抑制高糖下人视网膜血管内皮细胞增殖和VEGF表达。

AIM： To study the effects of different density of Rhizoma Zedoariae on the multiplication and apoptosis of human retinal capillary endothelial cells （HRCECs） and vascular endothelial multiplication factor （VECF） in high glucose environment. METHODS： Fresh eyeballs HRCECs extracted just after cornea transplanting operation were cultivated in vitro. The third or fourth generation healthy cells for this experiment were obtained, and divided into three groups： low glucose, high glucose, high glucose plus different density of Rhizoma Zedoariae group （60, 80, 100, and 120μg/mL）. MTT was employed to detect the multiplication of HRCECs, and the HRCECs VEGF through immune cells was observed.RESULTS： MTT comparison： there was no obvious difference between high glucose and low glucose groups （P0.05）, while there was significant difference between high glucose＋ and high glucose groups （P0.05）, and different density of glucose showed big difference. Immune cells： high glucose group had obvious affection compared with low glucose group, and there was significant difference between high glucose＋ group and high glucose group in their affection on VEGF（P0.05）, as well as in different density among high glucose group.CONCLUSION： Rhizoma Zedoariae can restrain the multiplication of HRCECs and VEGF in high glucose environment.