红景天苷与骨髓间充质干细胞联合应用对帕金森病大鼠的治疗作用

Treatment effect of salidroside combined with bone marrow mesenchymal stem cells on rats with Parkinson' s disease

目的:研究红景天苷与骨髓间充质干细胞(BMSCs)联合应用对帕金森病(PD)大鼠的治疗作用,为PD治疗提供实验基础。方法:采用贴壁法培养BMSCs,免疫荧光法检测细胞表面分子。利用MTT法分析10mg·L-1红景天苷对BMSCs增殖分化的影响。将60只大鼠随机分为假手术组、模型组、红景天苷治疗组、BMSCs治疗组、红景天苷和BMSCs联合治疗组,每组12只。在相对应的时间点检测各组大鼠行为学变化,取各组大鼠脑组织测定酪氨酸羟化酶(TH)mRNA表达水平。结果:成功分离提取表达干细胞标志物CD29和CD44细胞。红景天苷作用BMSCs后,BMSCs增殖较对照组更为旺盛且平台期较对照组延长。PD模型组大鼠旋转次数减少,与其他组比较差异有统计学意义(P<0.05);模型组大鼠行为改变明显,脑组织内TH mRNA表达水平降低。红景天苷和BMSCs联合治疗组大鼠行为学异常程度较轻,脑组织内TH mRNA表达水平高于其他组(P<0.05)。结论:红景天苷与BMSCs联合应用对PD大鼠的治疗作用优于单独应用红景天苷或BMSCs。

Objective To study the treatment effect of salidroside combined with bone marrow mesenchymal stem cells（BMSCs） on rats with Parkinson＇ s disease（PD）,and to provide experiment basis for curring Parkinson＇ s disease.Methods The BMSCs were cultured adherently,the cell surface molecules were detected by immunofluorescence.The effect of 10 mg·L-1 salidroside on the proliferation and differentiation of BMSCs was detected by MTT.60 Wistar rats were randomly divided into sham group,model group,salidroside treatment group,BMSCs treatment group,and salidroside and BMSCs combination therapy group.The behavior changes were detected at a specific time and the levels of tyrosin hydroxylase（TH） mRNA in brain tissue were detected.Results The setm cell markers CD29 and CD44 were expressed successfully.Compared with control group,the proliferation of BMSCs got bigger and the plateau got longer after treated with salidroside.The rotation number of PD model rats was the least compared with the other groups（P0.05）.The expression level of TH mRNA was decreased.The behavior abnormalities of PD model rats were improved and the level of TH mRNA was increased after treated with salidroside and BMSCs compared with the other groups（P0.05）.Conclusion The treatment efficacy of salidroside combined with BMSCs on PD rats is better than salidroside or BMSCs alone.