雪莲对小鼠腹腔巨噬细胞Fc和C_(3b)受体及红细胞免疫粘附功能的影响

The Effect of Saussurea Gossypiphora on Fc and C_3b Receptors of Macrophages from Abdominal Cavity in the Mouse and RBC Immune Adherence

检测巨噬细胞膜F_c和C_(3b)受体的活性及红细胞免疫粘附功能是选一步研究补益药增强机体免疫功能的重要手段。为探讨雪莲增强免疫作用的机制,本实验采用EA、YC花环形成以及红细胞C(3b)受体花环和免疫复合物花环形成等方法分别检测了雪莲和雪莲多糖对脾虚模型和氢化可的松琥珀酸钠(HCSS)抑制的小鼠腹腔巨噬细胞F_c和C_(3b)受体及红细胞免疫粘附功能的影响以及对胰酶损伤的小鼠腹腔巨噬细胞F_c和C_(3b)受体的修复作用。结果表明雪莲和雪莲多糖能提高脾虚模型和HCSS抑制的小鼠腹腔巨噬细胞EA和YC花环形成率及红细胞C_(3b)受体花环形成率和免疫复合物花环形成率,但二者在体外作用一小时,对胰酶损伤的小鼠腹腔巨噬细胞EA和YC花环形成率无显著影响,说明雪莲和雪莲多糖能增强脾虚模型和HCSS抑制的小鼠腹腔巨噬细胞膜F_c和C_(3b)受体及红细胞免疫粘附功能,对胰酶损伤的小鼠腹腔巨噬细胞F_c和C_(3b)受体,则无明显的修复作用。

Saussurea gossypiphora is a traditional Chinese medicine commonly used by Tibetan Veterinary for the purposes of promoting blood circulation、regulating menstruation and restoring the deficient, etc. Saussurea gassyphora and its polysaccharide have been demonstrated to be tonics, and they could raise the immunity of animals. For further study on the mechanism of their tonic effect, this experiment using EA-rosette. Yc-rosette, RBC C_(3b) receptor and RBC immune complex rosette techniques in vitro was conducted to investigate their effect on Fc receptors and C_(3b) receptor of macrophage from abdominal cavity in the mouse and RBC immune adherence. The result showed that the saussurea and its polysaccharide could markedly increase the activity of Fc and C_(3b) receptor of macrophages and RBC immune adherence from Pi-Xi (deficiency of the vital energy of spleen) mice and the mice suppressed by hydrocortisone, but within an hour, they could not increase activity of Fc and C_(3b) receptor of the peritoneal macrophage injured by trypsin in vitro.