宠物豚鼠隐孢子虫感染情况调查及其虫种鉴定

Survey on Cryptosporidium Infection and Identification of Species in Pet Guinea Pig

为了解宠物豚鼠隐孢子虫感染情况,用饱和蔗糖溶液漂浮法对郑州某宠物市场55份豚鼠粪便和免疫抑制后的30只豚鼠新鲜粪便样本进行检查。结果发现,隐孢子虫总感染率分别为12.7%和80%,卵囊呈近圆形,卵囊大小为5.3μm×4.7μm〔(5.0～5.5)μm×(4.4～4.9)μm〕,卵囊指数为1.11。基于18SrRNA基因位点,对调查中发现的7个隐孢子虫分离株进行PCR扩增,PCR-RFLP和测序结果显示,7个分离株均为维瑞隐孢子虫。宠物豚鼠隐孢子虫感染较为普遍,感染虫种是维瑞隐孢子虫,且豚鼠在免疫抑制后,其隐孢子虫感染率显著增高。

In order to investigate the infection status of Cryptosporidium in pet guinea pig,a total of 55 samples from pet market in Zhengzhou and 30 fresh stool samples from immunosuppressive guinea pigs were examined by Sheather＇s sugar flotation method. The results showed that the Cryptosporidiurn infection rates were 12.7% and 80% ,respectively. The oocysts were nearly cir-cular,with size of 5.3 μm×4.7μm and a length to width shape index of 1.11. PCR amplification of the 18S rRNA,PCR-RFLP and sequencing showed that all the seven isolates were Cryptospo-ridium wrairi ,indicating that the infection of Cryptosporidiurn in guinea pig was common after immunosuppression,and the infection rate was significantly higher.