思茅松AFLP分子标记体系的建立

Establishment of AFLP Molecular Mark System of Pinus kesiya var. langbianensis

采用改进的SDS法,提取思茅松胚乳DNA,获得较高质量的DNA样品,用Eco RI和Pst I 2种限制性内切酶完全酶切后,再经过预扩和选扩,从82对引物中筛选出24对带型分布均匀、多态性高且分辨力强的引物。扩增产物经聚丙烯酰胺凝胶电泳后,可获得清晰指纹图谱,重复性较好。建立的思茅松AFLP体系可用于后续研究。

High quality genome DNA of Pinus kesiya var. langbianensis was extracted from endosperm by the improved SDS method. After the DNA samples were completely digested by restriction enzymes of EcoRI and PstI and through pre-amplification and selective amplification, 24 pairs of primers out of 82 pairs with even band distri- bution, abundant polymorphism and high distinctiveness were selected. The distinct finger-printings of Pinus kesiya var. langbianensis with high reproducibility were obtained through electrophoresis of the amplified product. The es- tablishment of this AFLP mark system will be helpful to further study on Pinus kesiya var. langbianensis.