樟芝多糖的超滤分离及其免疫活性研究

Study on Ultrafiltration Separation and Immunocompetence of Polysaccharides from Antrodia

利用醇沉法和超滤法分离提取经发酵后的樟芝胞内胞外多糖，通过小鼠脾淋巴细胞转化增殖实验检测分离得到的各组分多糖的免疫活性。结果显示，经超滤分级处理后，樟芝胞内胞外多糖的总得率达18．18％，相对于乙醇沉淀法多糖得率提高了56．99％，显著的提高了樟芝胞内胞外多糖的得率；小鼠实验显示，利用超滤法获得的分子量大于1000kD、100～1000kD以及小于100kD的六个樟芝多糖胞内胞外组分均能显著地促进小鼠脾淋巴细胞的转化增殖作用，表明，超滤法能显著提高发酵后樟芝胞内胞外多糖提取产量，其操作简单、易行，不损害多糖活性。

Antrodia polysaccharide was separated by ethanol precipitation and ultrofiltrationr （UF） and the effects of Antrodia polysaccharide were investigated on immune function of mice. The result showed that the rate of Antrodia polysaccharide was 18.18% , increased by 56.99% compared to that by ethanol extracting. The yields ofAntrodia polysaccharides with molecular weights of 〉1000 kD （GFP100）, 100～1000 kD （GFP10） and 10～100 kD （GFP1） were 3.85%, 1.81% and 4.69%, respectively. All the polysaccharide components separated by UF can significantly increase the proliferation rate of mouse spleen lymphocytes. Conclusion： The method of ultrafiltration separation ofAntrodia polysaccharide was simple and feasible.