摘要
本文建立了HPLC测定茶饮料中阿斯巴甜含量的方法，采用色谱柱：THERMOC18（4．6×250mm，5μm），检测波长：210nm，流量：1．0mL／min，进样量：20μl，流动相：0．01mol／L磷酸二氢钾-乙腈为85：15。结果表明：阿斯巴甜在0～50μg/mL范围内呈现良好的线性关系，Y=52139X（0～50μg／ｍL）（R=0．9994）。平均回收率100．3％，RSD=0．33％。本方法简单，快速、准确、重现性好，可以用作茶饮料中阿斯巴甜的含量测定。
To establish a high performance liquid chromatography （HPLC） method for determination content of Aspartame in tea beverages ,20 μL samples were tested on a thermo C18 column using 0.01 mol-Lt potassium dihydrogen phosphate and acetonilrile （40：60） as mobile phase was with flow rate of 1.0 mL/min at 210nm.The results showed that Aspartame concentration bad good liner relationship within the range of 0～50 μg/mL （Y=52139X （0～50 μg/mL, R=0.9994）. The average recovery was 100.3% with RSD of 0.33%.The method was simple, fast, accurate, and precise, which could be used to determine content of aspartame in tea beverages.
出处
《现代食品科技》
EI
CAS
北大核心
2012年第12期1810-1812,共3页
Modern Food Science and Technology
