T细胞淋巴瘤患者外周血内皮祖细胞的变化及其临床意义

Change of EPCs in peripheral T-cell lymphomas and its significance

目的探讨内皮祖细胞(EPCs)在T细胞淋巴瘤非特异型(PTCL-NOS)患者外周血中的数量变化及其临床意义。方法采用流式细胞术检测25例PTCL-NOS患者及10例健康体检者外周血中EPCs的相对计数及绝对计数,观察患者治疗前后外周血中EPCs数量的变化,并分析其变化与患者临床特点的关系。结果 PTCL-NOS初治患者治疗前外周血EPCs相对及绝对计数较健康对照组高,PTCL-NOS初治患者治疗后外周血EPCs相对及绝对计数较治疗前降低,PTCL-NOS初治患者治疗后有效组外周血EPCs相对及绝对计数较难治组明显降低(P均<0.01)。Pearson相关分析显示,PTCL-NOS患者外周血EPCs计数与β2-微球蛋白、LDH、IPI评分呈正相关(r分别为0.523、0.476、0.421;P均<0.05);PTCL-NOS患者外周血EPCs绝对计数<20个/μL组与>20个/μL组生存曲线60周前有统计学差异(P<0.05)。结论 PTCL-NOS外周血EPCs细胞数与病情进展程度可能有一定的关系,EPCs计数有可能成为评价患者治疗效果及预后的指标之一。

Objective To investigate the number change of EPCs in peripheral blood of PTCL patients and its signifi- cance. Methods The relative number（RN） and absolute number（AN） of EPCs were determined by FCM method in 25 patients with PTCL-NOS and 10 normal controls. The change of EPCs in peripheral blood before and after treatment was ob- served, and the relation between this change and clinical character of patients analyzed. Results The number of EPCs in the peripheral blood of patients with PTCL-NOS before treatment was significantly higher than that in control group（ all P 〈 0.01 ）. The number of EPCs of PTCL-NOS patients after treatment was differently decreased compared with that before treatment（ all P 〈 0.01 ）. EPCs in effective group of PTCL-NOS initial treatment patients were lower than those in refracto- riness group. Pearson correlation analysis showed that there was positive correlation between the number of EPCs and LDH, 132-MG and IPI（ Correlation coefficients were 0. 523, 0. 476, 0. 421, respectively; all P 〈 0.05 ）. The survive curve had dif- ferencet of AN of EPCs between groups 〈 20 μL and 〉 20 μL before 60 weeks while without difference after 60 weeks. Conclusions EPCs may be correlated with progression of the disease in a certain degree. Dynamic observation with the level of EPCs may be used to evaluate the treatment outcomes and act as a prognostic marker for PTCL-NOS.