穿膜融合多肽TAT-N24对Jurkat细胞增殖的影响

Cell-permeable TAT-N24 Fusion Peptide Inhibits Proliferation of Jurkat Cells

目的观察穿膜融合多肽TAT-N24对急性T细胞白血病Jurkat细胞增殖的影响。方法以穿膜融合多肽TAT-N24处理Jurkat细胞,噻唑蓝(MTT)法观察细胞的生长情况,应用流式细胞仪检测Jurkat细胞周期进程。结果穿膜融合多肽TAT-N24处理急性T细胞白血病Jurkat细胞48 h,细胞生长受到抑制,随着多肽浓度的增加,抑制作用增强,表现出明显的浓度依赖性(P<0.05);48 h后细胞生长明显受抑,并随时间延长生长受抑更加明显,表现出时间依赖性(P<0.05)。空白对照组Jurkat细胞中G0/G1期细胞数为(41.91±1.96)%,S期和G2/M期细胞数分别为(52.16±1.76)%和(5.93±0.30)%;对照多肽组Jurkat细胞中G0/G1期细胞数为(46.38±2.31)%,S期和G2/M期细胞数分别为(44.22±1.69)%和(9.40±0.98)%;TAT-N24组Jurkat细胞中G0/G1期细胞数为(54.83±1.92)%,S期和G2/M期细胞数分别为(30.75±2.27)%和(14.42±0.68)%。结论融合多肽TAT-N24可以有效抑制急性T细胞白血病Jurkat细胞的增殖,阻滞其周期进程。

Objective To elucidate the inhibitory effect of cell-permeable TAT-N24 fusion peptide on Jurkat cell. Methods Jurkat cells were treated with cell-permeable TAT-N24. The cell growth was detected by MTI＂ method and the cell cycle progression determined by flow cytometer. Results TAT-N24 inhibited the cells in concentration- and time-dependent manners. The survival rate of cells exposed to TAT-N24 was lower than that of the control group after 48 h. The higher the concentration of TAT-N24,the lower the survival rate of the cells （P〈0.05）. The growth rate of the cells exposed to TAT-N24 began to decrease after 48 h, and this effect was significantly time-dependent （P〈0.05）. Cell cycle analysis revealed that the proportion of ceils in G0/G1, S and G2/M phases was （41.91 ±1.96） %, （52.16±1.76） % and （5.93±0.30） % respectively in the control group and was （46.38±2.31）%, （44.22_±1.69）%, （9.40±0.98）% respectively in the control peptide group. However in the TAT-N24 group, the proportion of cells in G0/G1, S and GJM phases was （54.83±1.92）%, （30.75±2.27）% and （14.42±0.68） % respectively. Conclusion TAT-N24 fusion peptide can restrain the growth of Jurka and induce the cell cycle arrest.