紫蓝素静脉注射的急性毒性实验

Acute Toxicity of Zilanine for Intravenous Injection in Rats

目的观察紫蓝素静脉注射对大鼠产生的急性中毒反应,以评价其安全性。方法 60只大鼠随机分为紫蓝素组(700 mg.kg-1.d-1)、3%碳酸氢钠溶液组、0.9%氯化钠溶液组,各20只。记录每只大鼠毒性反应的症状及开始和消失时间,连续观察14 d。结果①紫蓝素组给药2 h后大鼠全身皮肤呈紫色,大便呈紫蓝色,尿液呈黄褐色,第5天恢复正常。整个实验过程无大鼠死亡。②紫蓝素组大鼠体质量显著低于另外两组。③第1天紫蓝素组大鼠摄食量明显低于另外两组。④紫蓝素组大鼠肾脏脏器系数显著高于另外两组。⑤紫蓝素可引起部分大鼠肾脏髓质区域不同程度增大,皮质和髓质交界区域部分肾小管扩张;个别大鼠肾盂扩张,肾小管内有大量紫蓝色沉着。结论紫蓝素大鼠静脉注射给药的最大耐受量>700 mg.kg-1.d-1,主要表现为肾脏毒性。

Objective To observe the acute toxicity of excessive zilanine for single intravenous injection in rats and evaluate their safety. Methods Sixty rats were randomly and evenly divided into three groups： zilanine group （700 R285.5 ） ,3% NaHCO3 group,and 0.9% NaC1 group. The toxicity symptoms and the starting and ending time of toxicity response of the rats were recorded. The rats were observed and recorded for 14 days. Results In zilanine group,the skin of the rats became purple,their dejecta was indigo,and the urine presented filemot two hours after the injection of zilanine, but they returned to normal on day 5. No rats died in zilanine group. The body weight and weight increment of zilanine group were significantly lower than those in 3% NaHCO3 group and 0.9% NaC1 group. On day 1, food intake in zilanine group was significantly lower than that in 3% NaHCO3 group and 0.9% NaC1 group. The organ coefficient of kidney of zilanine group was significantly higher than that of 3% NaHCO3 group and 0. 9% NaC1 group. Zilanine caused pathological changes of the kidney in some rats. Conclusion The maximum tolerated dose of single intravenous injection was 700 R285.5 for zilanine. The main toxicity is kidney toxicity in rats.