欧洲型和美洲型PRRSV双重PCR检测方法的建立

A Duplex PCR Method for Detection of European Type and North American Type PRRSV

猪繁殖与呼吸综合征病毒(PRRSV)严重危害着养猪业的健康发展,给养猪业造成了巨大的经济损失。按照血清型和遗传性差异,PRRSV可划分为欧洲型和美洲型。欧洲型主要流行于欧洲地区,美洲型主要流行于美洲及亚太地区。我国每年从英国和法国等欧洲国家进口生猪占进口生猪总数的40%左右,但是在我国关于欧洲型PRRSV的报导却很少。为此,论文设计了2对能特异性扩增欧洲型和美洲型PRRSV的引物,建立并优化了可同时检测欧洲型和美洲型PRRSV的双重PCR方法。应用该方法能在3h内对样品进行检测并获得结果,本方法的建立对这两种病毒亚型的快速检测有十分重要的意义。

Porcine reproductive and respiratory syndrome virus （PRRSV） continues to be a threat, and causes huge economic losses in the swine industry. According to serotype and genetic differences, PRRSV can be divided into European type and American type. Europe type occurs mainly in Europe, the North American type occurs mainly in the Americas and Asia Pacific. China＇s annual import of live pigs from the United Kingdom,France and other European countries accounted ~or about 40% of the total number of imported live pigs, but rarely reports on the European type PRRSV. Eased on the whole genome sequences of PRRSV North American strains and PRRSV European strains in the GenBank, two pairs of specific prim- ers were designed to amplify the respective genes. The whole procedure can be completed within 3 hours, and this method is suitable for quick analysis and characterization of a large quantity of samples.