仔猪感染猪繁殖与呼吸综合征病毒的病毒定位研究

Study on Location of Virus in Piglets Infected with PRRSV

以猪繁殖与呼吸综合征病毒(Porcine reproduction and respiratory syndrome virus,PRRSV)天津分离株(PRRSV-SJ株)滴鼻感染10日龄～15日龄PRRSV阴性的健康仔猪,采用免疫组织化学法检测PRRSV在仔猪体内靶器官的动态分布,初步探讨PRRSV感染后在仔猪体内的病毒分布规律。结果表明,间接免疫组织化学法发现PRRSV-SJ株感染仔猪后1d～28d,均可在仔猪肺、淋巴结、扁桃体、气管、脾、肾等组织中检出PRRSV。感染后1d～7d,肺门淋巴结和扁桃体的病毒阳性细胞数量最多,阳性反应强。感染后14d～28d,脾脏的病毒阳性细胞数量多,阳性反应强。感染后1d,PRRSV就可在气管、肺门淋巴结和扁桃体检出。

The distributions of antigen were studies in 10-15day old piglets inoculated intranasally with porcine reproduction and respiratory syndrome virus（PRRSV） SJ strain by indirect immunohistochemical staining method and RT-PCR. The result showed PRRSV antigen was. found in lung, lymph node, tonsil, nasal mucosa, spleen, kidney in the test group by indirect immunohistochemical staining method at one day postinoculation up to twenty-eight days postinoculation. The most intense and widespread PRRSV positive ceils in bronchpulmonary lymph nodes and tonsil were at one day postinoculation up to seven days postinoeulation. The same result in spleen was at fourteen days postinoculation up to twenty-eight days postinoeulation. PRRSV antigen was found in nasal mucosa, bronchpulmonary lymph nodes and tonsil at one day postinoculation.