岷山红三叶异黄酮对去卵巢大鼠生长和免疫及抗氧化指标的影响

Effect of isoflavone in red clover on the growth and immune functions, and in the antioxidant indices in ovariectomized rats

为研究岷山红三叶异黄酮的促生长、免疫及抗氧化作用,选择80只SD(Sprague-Dawley)雌性大鼠,随机分为8组,每组10只,其中1～7组为去卵巢+红三叶异黄酮组,分别按320,160,80,40,20,10和0mg/(kg BW.d)的量灌胃红三叶异黄酮,第8组为假手术组,灌服等量生理盐水。结果表明,1)红三叶异黄酮对去卵巢雌性大鼠生长有一定的抑制作用,但不存在量效关系。2)低剂量的红三叶异黄酮具有提高大鼠T淋巴细胞转化率的作用,可以作为畜禽免疫增强剂,提高畜禽免疫力,以10mg/(kg BW.d)的添加量比较适宜。3)岷山红三叶异黄酮具有明显的抗氧化作用,适量(>80mg/kg BW.d)添加可降低血清CHO(total cho1esterol,总胆固醇)、HDL(high densi-ty lipoprotein cholesterol,高密度脂蛋白胆固醇)和LDL(low density lipoprotein cholesterol,低密度脂蛋白胆固醇)浓度,提高血清SOD(superoxide dismutase,超氧化物歧化酶)含量。4)岷山红三叶异黄酮具有弱的雌激素作用和较好的使用安全性。5)综合各指标,岷山红三叶异黄酮的适宜添加量为80mg/(kg BW.d)。

This study explored the influence of isoflavone in red clover（Trifolium pratense cv.Minshan） on animal growth,organism immunity and antioxidant functions.Eighty female Sprague-Dawley rats（Rattus norvegicus） at the 50-60 day old were randomly and evenly assigned to eight groups.The rats in groups 1 to 7 with the ovary excised were given red clover isoflavone daily at the rates of 320,160,80,40,20,10 or 0 mg/（kg·d） by gavage.The rats in group 8 were given the normal saline as the sham operation group.1） Red clover isoflavone inhibited the growth of the ovariectomized rats to some extent,but there was no dose-dependent effect.2） The transformation rate of T lymphocytes increased in response to the isoflavone at the lower doses,indicating that red clover isoflavone at the lower doses could be used as the immunopotentiator to improve the immunity of animals.The appropriate amount to add was 10 mg/（kg·d）.3） Red clover isoflavone had an obvious antioxidative effect.The correct addition（ 〉80mg/kg·d） may reduce the concentrations of CHO,HDL and LDL,but increase SOD activity in the serum.In addition,the isoflavone had a weak estrogen-like effect.The appropriate level for addition of the isoflavone was 80 mg/（kg·d） for the ovariectomized rats.