ISSR分子标记对9种狗牙根的鉴定分析

ISSR molecular markers analysis of 9 Bermudagrass cultivation varieties

狗牙根是全球最重要品种最丰富的暖季型草坪草种之一,种或品种间形态相同,传统的形态学方法、田间区试法等不能准确作出鉴定,ISSR分子标记被认为是目前准确高效的品种鉴定技术。本试验以9种常用狗牙根为对象,利用ISSR标记技术对9份材料间的遗传多样性进行了研究,构建了9份材料的指纹图谱。由ISSR标记分析结果可知,13条引物组合共获得54条清晰可辨的总条带,多态性条带数34条,平均每对引物扩增出4.1条带,多态性位点百分率为64.15%,品种间的遗传相似系数(GS)范围在0.547～0.962,变幅为0.415,说明供试材料具有比较丰富的遗传多样性。UPGMA聚类分析结果表明,GS=0.806时,9种常用狗牙根品种可聚为5类。黑杰克和南京狗牙根为第1类,普通狗牙根和瑞拉第2类,阳江狗牙根为第3类,摄政王为第4类,T-419、小矮人、老鹰草为第5类。利用ISSR引物UBC810和UBC857的扩增产物电泳图为基础构建的指纹图谱可以鉴定出9种狗牙根品种。

Bermudagrass are one of the most important and abundant warm-season turfgrass in the world.They are same on morphology between different species or varieties,and could not be identified accurately by traditional morphological method or field district test method.Inter-simple sequence repeat（ISSR） is considered to be the most accurate and efficient molecular marker technology currently on variety identification.In this study,we used ISSR on 27 Bermudagrass individuals to identify genetic diversity and construct Bermudagrass fingerprints of 9 accessions.Thirteen primers produced 54 polymorphic bands,of which 34 bands were found to be polymorphic,4.1 bands per primer on average.The percentage of polymorphic locus was 64.15% on average.The Nei＇s genetic similarity coefficient changed from 0.547 to 0.962,the range was up to 0.415.These demonstrated that test material had ample genetic diversity.Cluster analysis showed that all the accessions could be divided into five groups at GS 0.806： Blackjat and Nan jing Bermudagrass clustered into the first group,Riviera and Common Bermudagrass clustered into the second group,Yang jiang Bermudagrass clustered into the third group,Regent clustered into the forth group and Tifway（T-419）,Tifeagle,Tifdwarf clusterd into the fifth group.Research showed that 9 Bermudagrass varieties could be identified by fingerprints based on primer UBC810 and UBC857.The study offered theoretical and technical foundation for research genetic diversity and variety identification using ISSR technology,and could be widely used on variety identification,germplasm resources protection,hybrid parents selection,development and utilization.