摘要
目的观察蛋白CK-8在胃癌组织中的表达,并分析其临床病理学意义,为胃癌的早期诊断、预后评估提供临床参考。方法选用包括正常胃黏膜、癌旁、非典型增生、胃癌及淋巴结转移癌组织的组织芯片,通过免疫组织化学染色观察CK-8蛋白的表达情况,分析其在不同组织中的表达与临床病理的关系。结果在胃癌和淋巴结转移癌组织中,CK-8蛋白表达阳性率较正常胃黏膜、癌旁和非典型增生组织增加(P<0.01);在胃高分化腺癌、中分化腺癌和低分化腺癌中,随着分化程度的降低CK-8蛋白阳性表达逐渐升高(P<0.01)。结论 CK-8蛋白与胃癌的发生发展、分化程度及淋巴结转移有关。
Objective To observe the expression of CK-8 protein in gastric cancer tissue and analyze the significance of its clinical pathology,which would provide valuable data for clinical early diagnosis and evaluating the clinical result of gas tric cancer patients. Methods We analyzed the expression of CK-8 protein by immunohistochemistry in gastric tissue array including normal gastric mueosa tissue, carcinoma side tissue, atypical hyperplasia tissue, gastric cancer and lymphonode meta static carcinoma tissue. Meanwhile, the CK-8 protein expression relation with clinical pathology was evaluated in different gas trie tissue. Results The positive rate of CK-8 protein in gastric cancer tissue and lymphonode metastatic carcinoma tissue was higher than that in normal gastric mucosa, carcinoma side tissue and atypical hyperplasia tissue （P 〈 0.01 ）. The expres sion rate of CK-8 protein was gradually advanced with differentiation degree decreased in gastric well differentiated adenocar cinoma tissue, moderately differentiated tissue and poorly differentiated tissue （ P 〈 0.01 ）. Conclusion The CK-8 protein is related with occurrence and development, differentaiation degree and lymphaden metastasis of gastric cancer.
出处
《中南医学科学杂志》
CAS
2012年第6期555-558,共4页
Medical Science Journal of Central South China
基金
湖南省高校创新平台开放基金(No.10K052)
湖南省研究生科研创新项目(No.CX2010B380)
湖南省大学生研究性学习和创新性实验计划项目(No.CXSY-SJ-09007
No.CXSY-SJ-10192)
湖南省教育厅科研项目(No.11C1112)
衡阳市科技局科研项目(2011kj52
2011kj53)
关键词
胃癌
CK-8蛋白
发生发展
分化程度
淋巴结转移
Gastric cancer
CK-8 protein
occurrence and development
differentaiation degree
lymphadenmetastasis
