摘要
目的探讨大鼠肾移植术后急性排斥反应时TLR4 mRNA的表达及意义。方法建立大鼠肾移植模型,将大鼠分为健康对照组(6例),同基因移植组(30例),异基因移植组(C组,30例),采用实时荧光定量聚合酶链反应(PCR)技术分别于术后1、2、3、5及7 d检测受体大鼠血液及移植肾TLR4 mRNA的表达,同时检测其外周血肌酐,并在光镜下观察肾脏组织病理变化。结果异基因移植组TLR4mRNA的表达和血肌酐明显高于健康对照组和同基因移植组,且TLR4 mRNA表达在造模后第1天即明显升高,组织病理学也观察到排斥现象。结论 TLR4mRNA有助于早期诊断肾移植急性排斥反应的发生,可能作为一种新的监测标志物。
Objective To investigate the expression ofTRIA mRNA in predicting acute rejection after kidney transplantation. Methods After arthotopic kidney transplantation models were established in rats. The rats were divided into three groups,including normal control group（Group A, six rats ）,syngenic transplantation group（Group B,thirty rats） and allogenic transplantation group（Group C,thirty rats）. The TRL4 mRNA was measured by real-time fluorescent polymerasechain reaction（PCR） and and serum creatinine（Scr）levels were determined on day 1,2,3,5 and 7 after transplantation, the histopathology of kidney injury was observed by light microscope. Results Compared with normal control group and syngenic transplatantion group, the expression of TRL4 mRNA was obviously higher in allogenic transplantation group, aslo was in predicting. The expression ofTRL4 mRNA ascend higher 1 day later aider transplantation.. Light microscope observation indicates acute rejection injury in transplantation group. Conclusions The TRL4 mRNA help us discover acute rejection earlier, and may be used as a sensitive indicator in detecting acute rejection.
出处
《现代实用医学》
2012年第10期1093-1095,共3页
Modern Practical Medicine
基金
宁波市自然科学基金(2009A610160)
