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Pim-1抑制剂对巨噬细胞pNF-kBp65、iNOS，TNF-a表达的影响

Effect of Pim-] Kinase Inhibitor on the Expression of pNF-kB p65, iNOS and TNF-a in Macro-phage
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摘要 【目的】探讨Pim-1抑制剂对巨噬细胞pNF-kBp65、iNOS，TNF-a表达的影响。【方法】在脂多糖（LPS）刺激前给予不同浓度的Pim-1特异性抑制剂PIM-Inh，WesternBlot检测pNF-kBp65的表达，RT—PCR测定iNOS，TNF—d的表达，比较不同浓度抑制剂对pNF-kBp65、iNOS，TNF-a表达的影响。【结果】用不同浓度PIM-Inh预处理后，NF-kBp65、iNOS，TNF-a的表达明显下降（P〈0．05），且呈剂量依赖性，浓度100μmol／L时最为明显。【结论】PIMmInh特异性抑制剂可能抑制TLR4／NF-kB信号转导，降低TNF-a，iNOS的表达。 [Objective]To explore the effect of Pim-1 kinase inhibitor（PIM-Inh） on the expression of pNF- kB p65, iNOS and TNF-a in macrophage. [Methods] Before lipopolysaccharide（LPS） stimulation, different concentrations of PIM-Inh were given. The expression of pNF-kB p65 was detected by Western blot. RT-PCR was used to detect the expression of iNOS and TNF-a. The effects of different concentrations of PIM-Inh on the expression of pNF-kB p65, iNOS and TNF-a were compared. [Results] After the pretreatment with PIM- Inh 10μmol/L and 100/μmol/L, the expression of pNF-kB p65, iNOS and TNFa obviously decreased in a dose- dependent manner（ P d0.05）. The effect of PIM-Inh 100/μmol/L was most obvious. [Conclusion]PIM-Inh may inhibit TLR4/NF-kB signal transduction and reduce the expression of TNF-a and iNOS.
作者 申月明 曾亚
机构地区 湖南省长沙市中心医院消化内科
出处 《医学临床研究》 CAS 2012年第11期2049-2050,共2页 Journal of Clinical Research
基金 国家自然科学基金（项目批准号：81200277） 湖南省卫生厅课题（B2012-133）
关键词 巨噬细胞 NF-KB 分析 一氧化氮合酶 分析 肿瘤坏死因子 分析 macrophages NF-kappa B/AN nitric-oxide synthase/AN tumor necrosis factor/AN
分类号 R329.24 [医药卫生—人体解剖和组织胚胎学]
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参考文献8

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二级参考文献18

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医学临床研究
2012年 第11期

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