产甲烷菌的诱变及其对煤层气产出的影响

Mutagenesis of Methanogen and the impact of coalbed methane outputting

为获取高效降解褐煤产甲烷菌,以试验室超低温冰箱中保存的产甲烷菌为出发菌株,硫酸二乙酯(DES)作为诱变剂,粒度0.109～0.180 mm的褐煤为底物,在不添加培养基及营养成分的条件下进行诱变参数的优化,获取最佳诱变试验条件。试验结果表明,诱变的最佳试验条件为DES浓度1.8%、诱变时间30 min、诱变温度34℃;在此条件下经过40 d厌氧降解,产气量平均提高了40.00%。采用扫描电镜观察降解前后褐煤表面形态变化发现,与降解前相比,降解后褐煤表面变得疏松,孔隙和裂隙明显增多。

To obtain the Methanogen which can efficiently degrade lignite producing coalbed methane,the Methanogen saved in ultra cold storage freezer was used as the original strain,DES（Diethyl sulfate） was applied as the mutagen,and the lignite with the size range of 0.109-0.180 mm was taken as the substrate to conduct the experimental research.The DES mutagenesis parameters were optimized without adding any medium or nutrition constituent.The optimal experiment condition was obtained finally.The results show that best induced mutation conditions are DES dosage 1.8%,induced mutation time 30 min,induced mutation temperature 34 ℃;After 40 d biodegradation under the optimal experiment condition,the production of coalbed methane increases by 40.00%.Lignite surface morphology was analyzed with SEM（scanning electron microscopy） before and after the biodegradation,the results reveal that after biodegradation,the surface morphology of lignite becomes loose,meanwhile,the number of pore and fracture increase obviously.