亚洲百合和铁炮百合正反杂交亲和程度的差异性分析

Difference Analysis on the Degree of Positive and Negative Hybridization Affinity for Asian Lily and Longiflorum Lily

【目的】研究亚洲百合与铁炮百合杂交的亲和性,比较正反交组合在杂交亲和性上的差异。【方法】以亚洲百合‘Brunello’和铁炮百合‘新铁炮2号’配制正反交组合,利用荧光显微镜观察花粉在雌蕊中的生长动态。【结果】两个组合花粉均可在柱头上萌发,但花粉管在柱头乳突细胞、花柱通道细胞中会依次发生胼胝质反应。杂交后7—18 h,正交组合花粉管的平均生长速度一直比反交组合的快,18 h后反交组合花粉管的生长速度逐渐加快并超过正交组合。授粉后55 h正交组合花粉管到达花柱1/2处并停止生长,48 h时反交组合花粉管进入子房。正反交组合均表现柱头和花柱不亲和。【结论】正反交亲和性程度存在差异。采用切割花柱授粉和子房授粉结合胚培养可以克服杂交障碍,提高获得杂种胚的概率。

[Objective] The objective of this study was to study hybridization affinity of Asian lily and Longiflorum lily, and compare affinity differences existing in the obverse and inverse crosses. [Method] Asian lily ＇Brunello＇ and Longiflorum lily ＇Formolongi II＇ were used as the research materials and their reciprocal crosses were selected. The dynamic growth of the pollen in the pistil was observed by fluorescence microscopy. [Result] Pollen grains of the both cross combinations were able to germinate on the stigma. But, pollen tubes successively appeared callose reaction in the stigma papillae and style channel cells. Within 7-18 h after hybridization, the average growth rate of pollen tubes of cross combination was faster than the reciprocal cross combination. After 18h, the growth rate of pollen tubes of negative cross gradually accelerated and exceeded the positive cross. In the cross combination, pollen tubes reached half of style and stopped growth at 55 h after pollination. The pollen tubes of the reciprocal cross combination entered ovary at 48 h after pollination. The cross and reciprocal cross both showed hybrid incompatibility that occurred in stigma and style. [Conclusion] The extent of incompatibility in the reciprocal crosses had differences. Cutting style pollination, ovary pollination and embryo culture were able to overcome hybridization barriers and improve probability of hybrid embryos.