蜂王浆对雌性蜜蜂幼虫dynactin p62甲基化影响

Effect of Royal Jelly on the DNA Methylation of dynactin p62 in Female Honeybee Larvae

【目的】研究蜂王浆对雌性蜜蜂幼虫dynactin p62甲基化水平影响。【方法】以意大利蜜蜂(Apismellifera ligustica)和中华蜜蜂(A.cerana cerana)1日龄幼虫为试验材料,分别饲喂意大利蜜蜂蜂王浆和中华蜜蜂蜂王浆,并提取每组3日龄和6日龄幼虫样品的基因组DNA,利用Sequenom MassARRAY技术检测dynactinp62甲基化水平。【结果】饲喂异种蜂王浆可以更显著降低雌性蜜蜂幼虫dynactin p62整体甲基化水平;饲喂同一种蜂王浆,均为6日龄幼虫dynactin p62整体甲基化水平显著低于3日龄;2种蜂王浆对雌性蜜蜂幼虫dynactinp62各位点甲基化水平影响不同。【结论】2种蜂王浆对雌性蜜蜂幼虫dynactin p62整体甲基化水平和各位点甲基化水平影响不同,这说明不同蜂王浆具有不同的生物学效应。

[ Objective ] The objective of this study is to analyze the influence of royal jelly on DNA methylation of dynactin p62 in female honeybee larvae. [Method] One-day-old larvae ofApis mellifera ligustica and A. cerana cerana were used as materials, they were fed with royal jelly either from A. mellifera （RJM） or from A. cerana （RJC）, and genome DNA was extracted from 3-and 6-day-old larvae, then DNA methylation ofdynactinp62 was detected by Sequenom MassARRAY. [Result] The whole DNA methylation of dynactin p62 in female honeybee larvae decreased significantly after feeding with heterospecific royal jelly. The whole DNA methylation of dynactin p62 in 6-day-old larvae was,significantly lower than 3-day-old larvae after feeding with homologous royal jelly. The methylated sites of dynactin p62 in larvae were different after feeding with royal jelly （RJM and RJC）. [Conclusion] Royal jelly （RJM and RJC） had different effects on the whole DNA methylated and methylated sites ofdynactin p62 in female honeybee larvae. These results indicate that different types of royal jelly have different biological effects.