蒙古口蘑子实体的抗肿瘤活性

Anti-Tumor Activity of Tricholoma mongolicum Fruit Bodies

对蒙古口蘑子实体石油醚提取物及从中得到的麦角甾醇和麦角甾醇过氧化物的抗肿瘤活性进行研究,测定其对H22荷瘤小鼠抑瘤率、免疫器官指数及免疫因子含量的影响。同时探讨麦角甾醇和麦角甾醇过氧化物的细胞凋亡影响。结果表明:除了石油醚提取物上清低剂量组(50mg/(kg.d))外其他组均对肿瘤具有一定的抑制作用,其中石油醚提取物沉淀低剂量组(35mg/(kg.d))和麦角甾醇过氧化物低剂量组(5mg/(kg.d))抑瘤率分别达到69.61%和67.15%;各受试药组的胸腺指数与阳性对照组相比均有所增加,其中石油醚提取物沉淀高剂量组和麦角甾醇过氧化物的高剂量具有极显著性差异(P≤0.01);各组的脾指数没有规律性;所有石油醚提取物组的VD含量与正常对照组相近,麦角甾醇低剂量组和麦角甾醇过氧化物组与正常组对比具有极显著性差异(P≤0.01);所有受试药组的白介素-2(IL-2)含量明显增加,与阳性对照组和阴性对照组相比有极显著性差异(P≤0.01)。细胞凋亡实验结果表明麦角甾醇和麦角甾醇过氧化物均能诱导肝癌细胞(HepG2)凋亡,能够抑制HepG2细胞分裂,对HepG2细胞的早期细胞凋亡率分别为41.2%和42.33%。

The anti-tumor activity of petroleum ether extract and ergosterol and ergosterol peroxide extracted from Tricholoma mongolicum fruit bodies was studied by measuring their effects on tumor inhibitory rate,immune organ indexes and immune factor contents in H22-bearing mice.Besides,the effects of ergosterol and ergosterol peroxide on cellular apoptosis were investigated.Petroleum ether extract at 50 mg/（kg？d） did not have any tumor growth inhibitory activity.The tumor inhibitory rates of petroleum ether extraction residue at 35 mg/（kg？d） and ergosterol peroxide at 5 mg/（kg？d） were 69.61% and 67.15%,respectively.Each treatment group showed a higher thymus index compared with positive control group（cyclophosphamide） and highly significant differences were observed for high-dose（70 mg/（kg？d） petroleum ether extraction residue group and high-dose（10 mg/（kg？d） ergosterol peroxide group（P≤0.01）.However,there were irregular differences in spleen index between treatment and control groups.Serum VD content in petroleum ether extract groups was relatively close to normal control group,but ergosterol at 5 mg/（kg？d） and ergosterol peroxide at 5 mg/（kg？d） and 10 mg/（kg？d） revealed a highly significant difference compared with normal control group（P≤0.01）.Furthermore,a significant increase in serum IL-2 level was found for each treatment group compared with normal control group and there was a highly significant difference compared with positive control and negative control groups（P≤0.01）.The results of cellular apoptosis tests indicated that both ergosterol and ergosterol peroxide could induce the apoptosis of HepG2 cells and inhibit their division and resulted in an apoptotic rate at the early stage of 41.2% and 42.33%,respectively.