辅酶Q_9对人类血管内皮细胞氧化损伤的影响

Preventive Effect of Coenzyme Q_9 against Oxidative Damage in Human Vascular Endothelial Cells

目的:研究辅酶Q9对人类血管内皮细胞氧化损伤的影响。方法:采用MTT法构建H2O2诱导的人脐静脉内皮细胞HUVEC氧化损伤模型,研究辅酶Q9对HUVEC细胞氧化损伤的预防、修复作用及其作用机理。结果:质量浓度为100μg/mL的辅酶Q9对由H2O2诱导的HUVEC细胞的氧化损伤有预防和修复功能。抑制HUVEC细胞中ROS、MDA的增加是作用机制之一;同时,辅酶Q9预处理可有效减少H2O2对SOD酶的损伤,但对已损伤的SOD酶无修复作用。结论:辅酶Q9对H2O2造成的血管内皮细胞氧化损伤具有保护和修复功能。

Objective： To explore the protective effect of coenzyme Q9 against H2O2-induced oxidative damage in human vascular endothelial cells.Methods： An HUVEC（human umbilical vein endothelial cells） model of H2O2-induced oxidative damage was created by MTT assay and used to study the preventive effect and its mechanism of coenzyme Q9 against H2O2-induced oxidative damage.Results： Coenzyme Q9 at 100 μg/mL could exert preventive and repairing effects against H2O2-induced oxidative damage in HUVEC cells.The mechanism was partially due to that fact that the generation of ROS and MDA was inhibited by coenzyme Q9.Meanwhile,preventive treatment with coenzyme Q9 could effectively protect SOD against H2O2-induced damage but had no repairing effect.Conclusion： Coenzyme Q9 has protective and repairing effects against oxidative damage in human vascular endothelial cells.