血红素氧化酶-1在表浅性膀胱癌中的表达及其意义

Expression of heme oxygenase-1 in human superficial bladder cancer

目的探讨血红素氧化酶-1（HO-1）在人表浅性膀胱癌中的表达及意义。方法应用免疫组织化学链霉菌抗生物素蛋白一过氧化物酶（SP）法检测46例初发表浅性膀胱癌，其中术后复发组21例，术后无复发组25例以及8例正常膀胱组织中HO-1的表达，比较3组之间及膀胱癌不同病理分级之间的差异。结果46例膀胱癌组织中38例（82．6％）呈阳性表达，而8例正常膀胱组织中仅1例阳性表达，差异有统计学意义（P〈0．05），其中21例复发组膀胱癌95．2％阳性表达，26例无复发组膀胱癌中72．0％阳性表达，HO-1表达在有无复发组间差异有统计学意义（P〈0．05）。HO-1的阳性细胞表达在不同病理分级间差异有统计学意义（P〈0．05）。结论HO-1蛋白的表达可能与表浅性膀胱癌的发生、发展和复发有关。

Objective To investigate the expression profile of heme oxygenase-1 in human superfi- cial bladder cancer. Methods The expression of heme oxygenase-1 （ HO-1 ） was detected by using immu- nohistochemical streptavid-in-peroxidase （SP） method in 46 cases of initial onset of superficial bladder cancer [ including postoperative recurrence group （ n -- 21 ） and postoperative no-recurrence group （ n -- 25 ） 1 , and 8 cases of normal bladder tissues. A comparison was made between recurrence group and no-re- currence group, and among the pathological grades. Results The positive rate of HO-1 protein in human bladder carcinoma was 82. 6% , significantly higher than that in the normal bladder tissues （ 12. 5%, P 〈 O. 05 ）. There was significant difference in the expression of HO-1 between tissues of bladder carcinoma without recurrence and tissues of bladder carcinoma with recurrence （ P 〈 0. 05 ）. There was significant difference in the expression of HO-1 among the pathological grades （ P 〈 0. 05 ）. Conclusion The HO-1 expression may play an important role in the development, and recurrence of superficial bladder carcinoma.