自体脂肪间充质干细胞对大鼠肾局部缺血再灌注损伤的保护作用

Protective effect of autologous adipose-derived mesenchymal stem cells on renal ischemia reperfu- sion injury in rats

目的观察大鼠注射自体脂肪问充质干细胞（ADMSCs）对缺血再灌注（IR）损伤的保护作用。方法将24只成年雄性SD大鼠随机分为3组：A组（假手术正常组）、B组（IR＋培养基）、c组（IR＋ADMSCs），IR后1h尾静脉注射剂量为1．0×10。的白体ADMSCs。缺血时间1h，再灌注72h，接着处死大鼠观察。结果72h后，C组血清肌酐水平为（0．718±0．003）mg／dl，B组血清肌酐水平为（1．277±0．011）mg／dl，C组24h内尿量为（57．742±0．012）ml，B组24h内尿量为（23．665±0．027）ml，c组尿蛋白／肌酐的比值为1．217±0．033，B组比值为1．672±0．013，各指标两组比较差异有统计学意义（P〈0．05），c组肾组织异常程度相对于B组明显减轻；Westernblot法分析显示C组抗氧化标志物苯醌氧化还原酶基因（NQ01）和血红素氧合酶-1（HO-1）的蛋白表达水平显著高于B组（P〈0．05）。结论ADMSCs通过抑制氧化应激减轻IR诱导的肾损伤。

Objective To observe the protective effects of autologous adipose-derived mesenchy- real stem cells （ADMSCs） on the kidney in rats during renal ischemia reperfusion （IR） injury. Methods Adult male Sprague-Dawley （SD） rats （n = 24 ） were equally randomized into group A （sham control ） , group B （IR plus culture medium only） , and group C （IR plus intravenous administration of 1.0 x 106 autologous ADMSCs 1 h after IR）. The duration of ischemia was 1 h, followed by 72 h of reperfusion be- fore the animals were sacrificed. Results After 72 h, serum creatinine levels in groups C and B were （0. 718 _＋0.：003） and （ 1. 277 _＋ 0. 011 ） mg/dL, the urine volume in group C and B was （57. 742 _＋ 0.012） and （23.665 _＋0.027） mL, the ratio of protein and creatinine in group C and B was 1.217 _＋ 0. 033 and 1. 672 _＋ 0. 013 respectively, and the comparison of all above items showed statistically significan difference （ all P 〈 0. 05 ） ; Also, apparently, the renal pathohistological lesions were aggravated in group B as compared with those in group C. Western blotting showed notably higher NAD（P） H quinone oxi- doreductase 1 （NQO1） and heine oxygenase -1 （HO-1） protein expression in group C than in group B. Conclusion ADMSCs therapy minimized kidney damage after IR injury through suppressing oxidative stress.