维生素K3对尿路结石患者尿中骨桥蛋白浓度的影响

Effect of vitamin K3 on concentration of osteopontin in urine of patients with urolithiasis

目的观察维生素K3对尿路结石患者尿中骨桥蛋白浓度的影响。方法随机选取30例尿路结石患者为维生素K组，肌注维生素I（312mg／d，连续7d；收集用药前后的尿液标本；随机选30例正常人为对照组，收集尿液标本；利用Westernblot技术测定各尿液标本中的骨桥蛋白含量；比较尿路结石患者用药前后及与正常人尿液中骨桥蛋白含量的差异。结果维生素K组用药后比用药前尿液中骨桥蛋白含量高18．7％（P〈0．05）；用药前组比正常对照组尿液中骨桥蛋白含量高93．0％，但差异无统计学意义（P=0．036）；用药后组比正常对照组尿液中骨桥蛋白含量明显升高129．0％（P〈0．01）。结诊I临床廊用维毕素K可上调肾骨桥蛋白的嘉扶抑制结石的形甫．

Objective To investigate the effect of vitamin K on concentration of osteopontin in the urine of patients with urolithiasis. Methods Thirty cases of urolithiasis were treated with vitamin K3 injec- tion （12 rag/day for 7days） , and 30 healthy volunteers served as controls. The urine specimens were col- lected in patients before and after treatment and in controls. The concentrations of osteopontin in urine were determined by using Western blotting. Results The concentrations of osteopontin in urine were increased by 18. 7% after vitamin K3 treatment （P 〈 0. 05 ）. The concentrations of osteopontin in urine in vitamin K group before vitamin K administration were 93.0% higher than iri control group, but there was no signifi- cant difference （ P = 0. 36）. The concentrations of osteopontin in urine in vitamin K group after vitamin K administration were 129. 0% higher than in control group （ P 〈 0. 05 ）. Conclusion Vitamin K can clinically up-re：gulate the expression of osteopontin, and inhabit the formation of urolithiasis.