摘要
目的观察洛铂(LBP)对人胃癌细胞株(SGC-7901)增殖、凋亡及Metadherin(MTDH)基因表达的影响。方法采用噻唑蓝(MTT)比色法检测LBP对SGC-7901细胞的杀伤抑制作用;流式细胞仪检测SGC-7901细胞的凋亡;逆转录一聚合酶链反应(RT—PCR)和Westernblot法检测MTDHmRNA及其蛋白的表达。结果LBP可抑制胃癌SGC-7901的生长增殖,呈剂量-时间双效应关系(P〈0.05);LBP作用胃癌SGC-7901细胞48h后出现凋亡,且随着浓度的升高凋亡率也越来越高(P〈0.05)。不同浓度的LBP作用下的胃癌SGC-7901细胞中MTDHmRNA的表达明显减弱,且呈剂量梯度下降,0、5、10、20mg/L的LBP作用于胃癌SGC-7901细胞后的MTDH蛋白表达分别为:1.046±0.072、0.704±0.108、0.657±0.069、0.384±0.057,差异均有统计学意义(P〈0.05)。结论LBP可能通过抑制MTDH表达途径来抑制人胃癌SGC-7901细胞增殖并促进其凋亡。
Objective To investigate The effect of lobaplatin (LBP) on proliferation, apoptosis and Metadherin (MTDH) expression in human gastric cancer SGC-7901 cells, and the possible mecha- nism. Methods Methyl thiazol tetrazolium (MTT) method was used to detect antiprolifcrative rate of LBP on SGC-7901 cells. Flow cytometry was used to analyze the gastric cancer cell apoptosis. Reverse tran- scription-polymerase chain reaction (RT-PCR) and Western blotting were used to examine the expression of MTDH at mRNA and protein levels respectively. Results LBP significantly exhibited the cell growth inhi- bition in the SGC-7901 cells in a time- and concentration-dependent manner (P 〈 0.05 ). LBP significantly induced apoptosis in SGC-7901 cells in a dose-dependent manner (P 〈 0. 05). MTDH expression was sig- nificantly decreased by LBP at mRNA levels, and MTDH protein levels in SGC-7901 cells treated with 0, 5, 10 and 20 mg/L LBP were 1. 046 ±0. 072, 0. 704 ±0. 108, 0. 657 ±0. 069 and 0. 384 ±0. 057 respectively ( P 〈 0. 05 ). Conclusion LBP can inhibit proliferation of SGC-7901 cells and induce their apoptosis in vitroprobably by suppressing the MTDH expression pathway.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2012年第12期2479-2481,共3页
Chinese Journal of Experimental Surgery
基金
福建省科技厅自然科学基金资助项目(2011J01165)
福建省教育厅基金资助项目(JA11119)