洛铂对人胃癌细胞株SGC-7901增殖、凋亡及Metadherin基因表达的影响

Effect of lobaplatin on proliferation, apoptosis and expression of Metadherin in human gastric cancer SGC-7901 cells

目的观察洛铂（LBP）对人胃癌细胞株（SGC-7901）增殖、凋亡及Metadherin（MTDH）基因表达的影响。方法采用噻唑蓝（MTT）比色法检测LBP对SGC-7901细胞的杀伤抑制作用；流式细胞仪检测SGC-7901细胞的凋亡；逆转录一聚合酶链反应（RT—PCR）和Westernblot法检测MTDHmRNA及其蛋白的表达。结果LBP可抑制胃癌SGC-7901的生长增殖，呈剂量-时间双效应关系（P〈0．05）；LBP作用胃癌SGC-7901细胞48h后出现凋亡，且随着浓度的升高凋亡率也越来越高（P〈0．05）。不同浓度的LBP作用下的胃癌SGC-7901细胞中MTDHmRNA的表达明显减弱，且呈剂量梯度下降，0、5、10、20mg/L的LBP作用于胃癌SGC-7901细胞后的MTDH蛋白表达分别为：1．046±0．072、0．704±0．108、0．657±0．069、0．384±0．057，差异均有统计学意义（P〈0．05）。结论LBP可能通过抑制MTDH表达途径来抑制人胃癌SGC-7901细胞增殖并促进其凋亡。

Objective To investigate The effect of lobaplatin （LBP） on proliferation, apoptosis and Metadherin （MTDH） expression in human gastric cancer SGC-7901 cells, and the possible mecha- nism. Methods Methyl thiazol tetrazolium （MTT） method was used to detect antiprolifcrative rate of LBP on SGC-7901 cells. Flow cytometry was used to analyze the gastric cancer cell apoptosis. Reverse tran- scription-polymerase chain reaction （RT-PCR） and Western blotting were used to examine the expression of MTDH at mRNA and protein levels respectively. Results LBP significantly exhibited the cell growth inhi- bition in the SGC-7901 cells in a time- and concentration-dependent manner （P 〈 0.05 ）. LBP significantly induced apoptosis in SGC-7901 cells in a dose-dependent manner （P 〈 0. 05）. MTDH expression was sig- nificantly decreased by LBP at mRNA levels, and MTDH protein levels in SGC-7901 cells treated with 0, 5, 10 and 20 mg/L LBP were 1. 046 ±0. 072, 0. 704 ±0. 108, 0. 657 ±0. 069 and 0. 384 ±0. 057 respectively （ P 〈 0. 05 ）. Conclusion LBP can inhibit proliferation of SGC-7901 cells and induce their apoptosis in vitroprobably by suppressing the MTDH expression pathway.