新型复方凝胶剂对烫伤大鼠皮肤溃疡的治疗作用

New compound gel on enhancing healing of skin uclers in rats

目的观察胰岛素联合重组人表皮生长因子（rhEGF）的新型复方凝胶剂对大鼠皮肤溃疡的促愈合作用。方法采用水蒸气烫伤法在SD大鼠背部制备直径约1．2em的皮肤溃疡模型共40个，随机将溃疡分为新型复方凝胶剂治疗组（A组）、复方溶液治疗组（B组）、空白凝胶基质对照组（c组）。以治疗后不同时间点各组溃疡面积愈合百分率及组织病理学检查评价修复效果。结果治疗7d，A、B、C3组创面的愈合百分率分别为（50．23±5．67）％、（28．70±7．89）％、（12．6205．62）％；治疗11d，A、B、C3组创面的愈合百分率分别为（74．13±8．78）％、（57．14±6．52）％、（45．70±5．28）％；治疗15d，A、B、C3组创面的愈合百分率分别为（92．99±5．60）％、（82．61±5．89）％、（68．67±6．03）％，伤后各时间点A组创面愈合率均显著高于其余各组，差异均有统计学意义（P〈0．05）。结论胰岛素联合rhEGF的新型复方凝胶剂对大鼠烫伤皮肤溃疡有显著的促愈合作用，效果优于复方溶液和空白凝胶基质。

Objective To investigate the effect of new compound gel of insulin plus recombinant human epidermal growth factor （rhEGF） on the healing of skin ulcers in rats. Methods The steam burn method was used to prepare 40 skin ulcers about 1.2 cm in diameter on the back of rats. These skin ulcers were randomly divided into 4 groups ： new compound gel treatment group （ group A ）, compound solution treatment group （group B）, blank gel treatment group （group C）. The effect on ulcer healing was assessed by the rate of ulcer healing and histopathologieal changes of each group. Results After treatment for 7 days, the rate of ulcer healing in group A, group B and group C was （ 50. 23 ± 5.67 ） % , （ 28.70 ± 7. 89） %, and （ 12. 62± 5.62 ） % respectively. After treatment for 11 days, the rate of ulcer healing in group A, group B and group C was （74. 13 ~8.78）% , （57.14 ---6.52）% , and （45.70 ~5.28）% re- spectively. After treatment for 15 days, the rate of ulcer healing in group A, group B, and group C was （92. 99 ± 5.60）%, （82. 61± 5.89）%, and （68. 67 ±6. 03）% respectively. At any time point after treatment, the rate of ulcer healing in group A was higher than other groups （ P 〈 0. 05 ）. Conclusion New compound gel of insulin plus rhEGF ~an promote the healing of skin ulcers in rats.