人类肠道病毒74、80和87型山东地方株的鉴定及基因特征分析

Identification and Molecular Characterization of Human Enteroviruses Type 74,80,and 87 Isolated in Shandong Province,China

随着基于VP1序列分析的分子定型方法的推广应用,许多新型人类肠道病毒(Human enterovirus,HEV)陆续被发现。本研究通过对1989～2010年山东省急性弛缓性麻痹(Acute flaccid paralysis,AFP)监测系统分离到的非脊髓灰质炎肠道病毒进行VP1完整编码区的序列扩增及分子定型,共发现1株EV74,3株EV80和1株EV87。同源性分析显示EV74、EV80和EV87山东分离株与原型株的核苷酸同源性分别为81.4%,76.4%～81.7%以及80.3%。系统发生学分析显示山东地方株与国内外其他分离株的亲缘关系均较远。这是在中国大陆地区首次发现EV74和EV87,其中EV87山东分离株为全球第2个鉴定出的分离株。这3种新型HEV在AFP监测系统中的分离率较低,提示尚未在我国导致大规模流行。

An increasing number of new types of human enteroviruses（HEV） have been identified with the application of molecular typing method based on VP1 sequence analysis.In this study,the non-polio enteroviruses（NPEV） isolated from acute flaccid paralysis（AFP） cases in Shandong Province,China were typed via molecular typing method,and 1 EV74,3 EV80 and 1 EV87 strains were identified.Homologous comparison revealed EV74,EV80 and EV87 Shandong strains had 81.4%,76.4%-81.7%,and 80.3% VP1 identities with prototype strains.Phylogenetic analysis suggested a remote genetic distance with other strains.This is the first report of EV74 and EV87 in China's Mainland,and the low isolation rate in AFP surveillance suggested these three serotypes has not been the predominant viruses in China.