摘要
目的:建立卵白蛋白(ovalbumin,OVA)诱发的变应性鼻炎(allergic rhinitis,AR)豚鼠模型,观察其临床症状、病理学和免疫炎症反应有关指标的变化特点。方法:实验豚鼠分为正常组和模型组。模型组动物经腹腔注射0.8ml的OVA混悬液进行全身致敏,并经双侧鼻腔按每侧20μl的OVA生理盐水溶液(60mg/ml)滴鼻进行局部致敏,致敏期限为21d;间隔1周后,以OVA生理盐水溶液(60mg/ml)滴鼻激发,每天1次,连续7d,而后改为两天1次,连续14d,剂量均为50μl/kg。正常组动物以生理盐水代替OVA悬液或OVA生理盐水溶液,用法用量与模型组相同。激发阶段,动态观察各组豚鼠喷嚏、抓鼻、眼泪、鼻涕及呼吸困难症状变化;激发期结束,动物取血制备血清用于IgE含量检测,剥取鼻黏膜用于组织病理学观察及组胺含量测定。结果:与正常组比较,模型组豚鼠出现典型的变应性鼻炎症状,喷嚏数增加并伴有严重抓鼻现象,血清IgE及鼻黏膜组胺含量均明显升高(P<0.01);鼻黏膜组织出现嗜酸粒细胞浸润、血管扩张及上皮脱落等典型的炎性病理变化。结论:OVA诱发的变应性鼻炎豚鼠模型,可较好模拟人类变应性鼻炎的临床特征,该模型是研究变应性鼻炎机制及治疗药物的良好模型。
OBJECTIVE:To establish an allergic rhinitis model induced by ovalbumin (OVA) in guinea pigs and identify the characteristics in clinical symptoms,pathology and immunology. METHODS:Hartley guinea pigs were randomly divided into two groups:normal group and model group. The model animals were sensitized systematically with 0.8 ml suspension containing OVA,aluminium hydroxide gel and physiological saline (0.2 mg:0.3 ml:0.5 ml),by intraperitoneal injection and topically sensitized with 60 mg/ml ovalbumin dissolved in physiological saline by intranasal instillation,20 μl each nostril,for 21 days. After an interval of one week,the sensitized animals were challenged with 60 mg/ml ovalbumin,50 μl/kg each nostril,once daily for a week and one time every two days for two weeks. The normal animals were treated with physiological saline instead of the OVA suspension and solution for intraperitoneal injection and intranasal instillation. The symptoms of allergic rhinitis,such as sneezing,nose rubbing,lacrimation, nasal congestion and rhinorrhea were observed after every challenge. The serum and the nasal mucosas were prepared for the detection of IgE and histamine contents and histological examination. RESULTS:Compared with normal group animals,guinea pigs in the model group developed typical clinical symptoms,indicated by the increasing numbers of sneezing and nose rubbing,higher levels of the histamine and serum IgE (P0.05). Pathological alterations in the nasal mucosa included eosinophil infiltration,vasodilatation and epithelial exfoliation. CONCLUSION:An OVA-induced model of AR in guinea pigs was established,mimicking the clinical characteristics of human AR and can be used in the mechanism study and the drug development for AR.
出处
《癌变.畸变.突变》
CAS
CSCD
2012年第6期440-443,共4页
Carcinogenesis,Teratogenesis & Mutagenesis
关键词
卵白蛋白
变应性鼻炎
豚鼠
模型
ovalbumin
allergic rhinitis
guinea pig
model