冷冻载体浓度对复苏卵母细胞存活率的影响及蔗糖

The effects of the different combinations of freezing carrier and sucrose concentration on the oocyte survival after thawing

目的:探讨MⅡ卵母细胞玻璃化冷冻和复苏过程中不同冷冻载体和不同蔗糖浓度解冻对卵母细胞存活率的影响。方法:收集2009年6月至2012年5月在成都市锦江区妇幼保健院生殖医学中心常规IVF治疗周期第1天没有受精的MⅡ卵母细胞853枚,进行玻璃化冷冻后复苏。按照实验过程中所用不同冷冻载体和不同解冻方法分为4组。A组79枚,载体为冷冻环,采用常规方法解冻;B组580枚,载体为半麦管,采用常规方法解冻;C组121枚,载体为冷冻环,采用改良法解冻;D组73枚,载体为半麦管,采用改良法解冻。将卵母细胞复苏2h后观察,对各组卵母细胞存活率进行了比较。结果:C组卵母细胞存活率(97.52%)显著高于A组(72.15%)、B组(52.24%)和D组(64.38%)(P<0.01);A组卵母细胞存活率高于B组,差异显著(P<0.05);其余各组间卵母细胞存活率差异无统计学意义(P>0.05)。结论:采用冷冻环冷冻及改良法解冻能提高成熟卵母细胞存活率。

OBJECTIVE：To investigate the effects of different combinations of sucrose concentration and freezing carrier on oocyte survival after thawing. METHODS：853 failed-fertilization oocytes after conventional IVF were collected and frozen with vitrification method in Center for Reproductive Medicine,Chengdu Jinjiang Hospital for Women and Child Health Care from June 2009 to May 2012. All oocytes were divided into four groups;group A：79 oocytes were vitrified with the cryoloop carrier and thawed using the conventional method;group B：580 oocytes were vitrified with the hemi-straw carrier and thawed using the conventional method;group C：121 oocytes were vitrified with the cryoloop carrier and thawed using the modified method;group D：73 oocytes were vitrified with the hemi-straw carrier and thawed using the modified method. After thawing for 2 h,the oocyte survival rates were compared among the groups mentioned above. RESULTS：The rate of oocyte survival in group C （97.52%） was significantly higher than those in group A （72.15%）,group B （52.24%） and group D （64.38%） （P 0.01,respectively）. The rate of oocyte survival in group A was significantly higher than that in group B （P 0.05）. No statistical difference in the rate of oocyte survival was found between other groups （P0.05）. CONCLUSION：The survival rate of MⅡ oocytes could be improved when oocytes were vitrified with the cryoloop carrier and thawed using the modified method.