浊漳河水体中有机污染物的遗传毒性研究

Study on genotoxicity of organic pollutants in the Zhuozhang River

目的:评价浊漳河水体有机污染物对人外周血淋巴细胞的遗传毒性。方法:采用单细胞凝胶电泳试验(彗星试验)对浊漳河水体3个不同样点的有机提取物所致的人外周血淋巴细胞的DNA损伤进行研究。结果:在一定剂量范围内,各水样有机提取物均能引起人外周血淋巴细胞不同程度的DNA损伤,并随剂量的增高而加重,与阴性对照组相比,差异均具有统计学意义(P<0.01);以每管100ml剂量组进行多重比较表明,各采样点水样对人血淋巴细胞DNA损伤程度从大到小依次为暴马、黄碾、石梁。结论:浊漳河水体有机污染物对人外周血淋巴细胞DNA有断裂损伤作用,彗星试验技术的应用有助于水环境监测以及水中有机提取物遗传毒性的研究。

OBJECTIVE：The genotoxicity on human peripheral lymphocytes by organic pollutants in the Zhuozhang River was investigated. METHODS：The comet assay was conducted to detect DNA damage induced by the organic extracts of three typical sample sites. RESULTS：Within certain concentrations,the DNA damage in human peripheral lymphocytes was induced by the substances tested and increased with the dose of the organic extracts. Compared with the negative control group,there were significant differences（P0.01）. The differences of DNA damage were also shown in different samples by multiplex comparison at the dose of 100 ml/tube,with decreasing activity as follows,BaomaHuangnianShiliang. CONCLUSION：The organic pollutants in Zhuozhang River caused DNA damage in human peripheral blood lymphocytes. This study also demonstrated that comet assay could be successfully applied to the genotoxicity monitoring of water organic extracts.