HSP90β在胃腺癌中的表达及其临床病理意义

Expression of HSP90β in gastric adenocarcinoma and its clinical pathological significance

目的探讨热休克蛋白90β(HSP90β)在胃腺癌中的表达及其与肿瘤细胞增殖和临床病理参数之间的相关性,并分析其与患者预后的关系。方法应用免疫组织化学PV-9000二步法检测60例胃腺癌组织和20例胃炎组织中HSP90β和细胞增殖因子Ki-67的表达,采用χ2检验分析各临床病理参数间的关系,用Φ系数分析HSP90β和Ki-67的相关性,并对60例胃腺癌患者进行生存分析。结果 HSP90β和Ki-67在胃腺癌中的表达高于在胃炎组织中的表达(P<0.05),且低分化胃腺癌中阳性率远高于其在高中分化胃腺癌中的阳性率(P<0.05);HSP90β与Ki-67在胃腺癌中的表达呈正相关(Φ=0.368,P<0.05);HSP90β表达阳性患者的生存周期小于阴性表达者(P<0.05)。结论 HSP90β在胃癌中表达明显上调,其表达与Ki-67呈正相关,并提示预后不良。

Objective To explore the expression of heat shock protein 90β （HSP90β） in gastric adenocarcinoma and its correlation with proliferation and clinicopathological parameters of the tumor cells, and to analyze the relationship of HSP90β with the patients＇ prognosis. Methods Use immunohistochemistry PV-9000 two-step method to detect the ex- pression of HSP90β and Ki-67 in 60 cases of gastric adenocarcinoma and 20 cases of gastritis, the χ2 test was used to analyze the relationship between clinicopathological parameters, the Ф coefficient was used to discuss the correlation be- tween the HSP90β and Ki-67, and survival analysis was performed on 60 patients with gastric cancer. Results Expres- sions of HSP90β and Ki-67 in gastric adenocarcinoma were higher than those in gastritis tissues （ P 〈 0.05 ）, and the positive rate in poorly differentiated gastric adenocarcinoma was much higher than that in well and moderately differenti- ated gastric adenocarcinoma （P 〈 0.05 ） ;there was a positive correlation of expression between HSP90β and Ki-67 in gastric adenocarcinoma（ Ф = 0. 368, P 〈 0.05 ）. The survival period of patients with HSP90β positive expression was less than that of patients with negative expression. Conclusion HSP90β is significantly up-regulated in gastric carcino- ma. Its expression is positively correlated to Ki-67 and heralded a poor prognosis.