血清瘦素及受体基因Gln223 Arg多态性与胃食管反流病的相关性

Relations between serum leptin and leptin receptor Gln223Arg polymorphism with gastro-esophageal reflux disease

目的探讨人外周血血清瘦素及瘦素受体(LEPR)基因Gln223Arg多态性与胃食管反流病(GERD)的相关性。方法根据GERD症状诊断问卷联合胃镜检查确诊GERD,选取具有完整临床资料的GERD患者62例,分为反流性食管炎组(RE组)、非糜烂性反流病组(NERD组),同时选取健康体检者63例为对照组。采用酶联免疫法(ELISA)定量检测血清瘦素;采用限制性片段长度多态性聚合酶链反应法检测血清瘦素受体基因Gln223Arg多态性。结果 RE组、NERD组瘦素水平均明显高于对照组(P<0.05);RE组炎症的严重程度与瘦素水平呈正相关。RE组、NERD组瘦素受体基因Gln223Arg的GA+AA基因型频率和A等位基因频率均明显高于对照组(P<0.05)。GERD患者中GA+AA基因型的瘦素水平明显高于GG基因型(P<0.05)。结论 GERD的发生与血清瘦素水平升高及瘦素受体基因Gln223Arg多态性相关。

Objective To investigate the relations between serum leptin and leptin receptor （LEPR） Gln223Arg poly- morphism with gastro-esophageal reflux disease（GERD）. Methods 62 patients with GERD were selected according to reflux disease questionnaire and gastroscope inspection and divided into the reflux esophagitis （RE） group and non-ero- sive reflux disease （NERD） group. In addition, 63 healthy subjects were selected as the control group. The serum leptin levels were determined by ELISA, and LEPR Gln223Arg polymorphisms were determined by polymerase chain reaction-restriction fragment length polymorphisms assay. Results The levels of leptin in RE group and NERD group were both significantly higher than in control group （ P 〈 0.05 ） ; the degree of inflammation in RE group was positively correlated with leptin level. GA ＋ AA genotype and A allele frequency of LEPR gene in RE group and NERD group were significantly higher than those in control group （ P 〈 0.05 ）. The leptin in patients with GA ＋ AA genotype was significantly higher than that in patients with GG genotype （P 〈 0.05 ）. Conclusion The serum leptin and the LEPR Gln223Arg polymorphism are associated with GERD.