不同种雏鸭建立鸭乙肝病毒感染模型及抗病毒效果的实验

Experimental Study on Duck Hepatitis B Virus Infection Model by Different Kinds of Ducklings and Anti-viral Effect

目的探讨不同种雏鸭建立鸭乙肝病毒感染模型的影响因素,观察应用该模型抗病毒的效果。方法采集鸭血清,应用PCR方法定性检测鸭血清中病毒DNA;定量PCR方法检测鸭血清中病毒DNA载量变化;用抗病毒药物处理,观察其在鸭DHBV感染模型中的抗病毒效果。结果不同种鸭DHBV自然感染率不同,樱桃谷鸭为8.75%,湖北麻鸭两个批次分别为17.80%和10.68%;静脉注射和腹腔注射两途径均能致雏鸭感染DHBV,静脉注射感染率80%,腹腔注射感染率65%;鸭感染DHBV后,体内病毒载量维持在106～108copies/mL,可持续20 d以上;抗病毒药物处理后,在不同DHBV模型中其抗病毒效果变化趋势一致。结论鸭的种类和人工感染途径可影响DHBV感染率;雏鸭感染DHBV后其体内有持续性的病毒血症;DHBV感染模型是药物抗病毒研究较好模型。

Objective To explore the factors in duck hepatitis B virus infection model by different kinds of ducklings and observe the antiviral effect in models. Methods Collecting duck sera, qualitative detecting viral DNA by PCR method and detecting the changes of viral DNA load by the quantitative PCR method in duck sera. Observing the antiviral effects in duck DHBV infection models after antiviral drug treatment. Results Natural infection rate of duck DHBV is different, Cherry Valley duck for 8.75%, two batches in Hubei sheldrake were 17. 80% and 10. 68% respectively. Both intravenous and intraperitoneal injection can cause ducklings infected with DHBV, infection rates of two ways were 80% and 65% respectiviely. Ducks infected with DHBV, the viral load is maintained at 106 ~ lOs copies / mL and persist for more than 20 days. Changing trends of antiviral effect in different DHBV models are coincident after treating by the antiviral drugs. Conclusion Duck species and artificial routes of infection can affect DHBV infection rate, in vivo the viremia of duck is stably persistent after DHBV infection. Therefore, DHBV infection model is an available model in antiviral drug screening.