三种麻醉方法在骨科动物实验中效果比较

Comparing of Three Anesthetic Methods during Orthopedic Animal Experiments

目的比较3种麻醉方法在骨科动物实验中的效果,从而得出最佳方法。方法将80只实验动物随机分为3组,A组(速眠新II肌肉注射组),B组(0.6%戊巴比妥钠静脉注射组),C组(速眠新II肌肉注射+0.6%戊巴比妥钠静脉注射联合用药组),在骨科动物实验中分别进行麻醉、手术,比较3组的麻醉效果,观察其诱导期、麻醉期(麻醉维持时间)、苏醒期、麻醉效果、死亡率等。结果 A组麻醉的诱导期长,效果不好;B组麻醉的诱导期短,但麻醉效果不理想,死亡率高;C组麻醉的诱导期短,麻醉效果好,安全。结论 C组诱导期短,麻醉效果好,安全。无论在实验动物的伦理道德方面,还是在保证动物实验质量方面都是最佳选择。

Objection To determine the optimal anesthetic method, our study compared the effect of three anesthetic methods in orthopedic animal experiments. Methods 80 experimental animals were randomly divided into three groups, group A was given with sumianxin II by intramuscular injection, group B was administrated with 0. 6% pentobarbital sodium by intravenous injection, and group C was given with 0. 6% pentobarbital sodium by introvenous injection combined with sumianxin II by intramuscular injection. Anesthesia was performed in orthopedic animal experiments surgery. Induction period of anesthesia （ maintenance of anesthesia time） , the recovery period, the anesthetic effect, mortality rates were observed after anesthesia. Results In group A, anesthesia induction period was long, and the anesthesia effect was not very good; In group B, anesthesia induction period was short, but the effects of anesthesia was bad and the mortality rate was high; however, induction period of group C was short and safety. Conclusion Pentobarbital sodium combined with sumianxin II is a good anesthetic method compared with pentobarbital sodium or sumianxin II respectively. Its induction period is short and safety. Therefore, it is an excellent and safe method to anesthetize animal in orthopaedic using Sumianxin II combined with pentobarbital sodium.