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胃癌细胞株中NPRA基因启动子区CpG岛的甲基化分析 被引量：1

Methylation status in the NPRA promoter in gastric cancer cell lines
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摘要 目的NPRA-cGMP信号通路系统可激活cGMP依赖的蛋白激酶,活化的蛋白激酶调控离子转运蛋白和转录因子的表达,从而最终影响细胞生长、凋亡、增殖和炎症反应。本研究旨在研究NPRA基因在胃癌细胞中的甲基化状态及其对下游mRNA表达的影响。方法应用亚硫酸氢钠处理基因组DNA后PCR并测序,我们分析了6株胃癌细胞株NPRA基因的甲基化状态及去甲基化试剂对下游mRNA表达的影响。结果在胃癌细胞中发现NPRA基因启动子区存在甲基化畸变并导致下游mRNA表达减少或沉默。去甲基化试剂可逆转mRNA表达沉默。结论胃癌细胞中存在NPRA基因甲基化畸变。 Objective NPRA is ANP binding protein that catalyze the synthesis of the intracellular second messenger cGMP. Research suggests that ANP have useful antieancer properties and part of their mechanism of action appears to be mediated by cGMP. It remains to clarify the role of NPRA to mediate ANP effect. In this study, we analyzed NPRA epigenetic inactivation, bio- logical effects, and prognostic significance in gastric cancer. Methods Methylation status was evaluated by bisulfite sequencing. We have identified hypermethylation in the promoter region of NPRA in six gastric cancer cell lines, HGC-27, MGC80-3, NCI- N87, AGS, BGC-823, SGC-7901. Results NPRA was silenced or down-regulated in all of six gastric cancer cell lines. Expression levels were restored by exposure to demethylating agents. Conclusion Methylation of the NPRA is frequent in gastric cancer.
作者 陈华云 杨东杰 张常华 宋武 彭建军 何裕隆
机构地区 中山大学附属第一医院胃肠外科
出处 《解剖学研究》 CAS 2012年第5期355-357,共3页 Anatomy Research
关键词 胃癌 甲基化 表观遗传学 胃癌细胞株 NPRA Gastric cancer Methylation Gastric cancer cell lines NPRA
分类号 R735.2 [医药卫生—肿瘤]
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参考文献8

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同被引文献6

  • 1Stryjewski P J,Nessler B,Cubera K,et al.Natriuretic peptides.History of discovery,chemical structure,mechanism of action and the removal routes.Basis of diagnostic and therapeutic use[J].Przegl Lek,2013,70(7):463-467.
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  • 3Kong X,Wang X,Xu W,et al.Natriuretic peptide receptor a as a novel anticancer target[J].Cancer Res,2008,68(1):249-56.
  • 4Wang X,Raulji P,Mohapatra SS,et al.Natriuretic peptide receptor a as a novel target for prostate cancer[J].Mol Cancer,2011,10:56.
  • 5Wang JC,Fiscus RR.Essential roles of the nitric oxide(no)/cGMP/protein kinase G type-Iα(PKG-Iα)signaling pathway and the atrial natriuretic peptide(ANP)/cGMP/PKG-Iαautocfine loop in promoting proliferation and cell survival of OP9bone marrow stromal cells[J].J Cell Biochem,2011,l12(3):829-839.
  • 6张佳,赵志龙,陈敬信,张明鑫,胡海舰,祖超,沈辉,王健生.NPR-A在胃腺癌中的表达及意义[J].现代肿瘤医学,2013,21(12):2744-2746. 被引量：2

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解剖学研究
2012年 第5期

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