Galectin-1与VEGF、CD105在宫颈癌组织中的表达及其临床意义

Expression and clinical significance of Galectin-1,VEGF,CD105 in cervical cancer

目的:研究宫颈癌组织中CD105和Galectin-1、VEGF的表达及其与血管生成的关系,探讨其在宫颈癌侵袭与转移中的作用。方法:应用免疫组织化学法检测Galectins-1与VEGF、CD105在30例正常宫颈组织及40例宫颈癌组织中的表达,并分析三者在宫颈癌发生及发展过程中的作用及相互关系。结果:40例宫颈癌组织中Galectin-1和VEGF的阳性表达率分别是70%和75%,30例正常宫颈组织中的阳性表达率分别为10%和20%。宫颈癌组织中Galectin-1和VEGF的阳性表达率明显高于正常宫颈组织,差异有统计学意义(P<0.05);宫颈癌组织阳性表达均与不同的组织学分化程度、FIGO分期和淋巴结转移相关(P<0.05),而与患者年龄、组织大体类型无关。CD105作为新生血管的标记物测得的MVD值与宫颈癌的临床分期和淋巴结转移相关(P<0.05),与患者的年龄、大体类型和组织学分化程度无关。宫颈癌组织中,Galectin-1与VEGF的阳性表达呈正相关(P=0.043、r=0.321);Galectin-1及VEGF的阳性表达程度均与MVD呈正相关(P=0.003、r=0.453)(P=0.016、r=0.379)。结论:Galectin-1促肿瘤血管生成的作用可能是通过上调VEGF的表达来实现的,从而参与宫颈癌的侵袭与转移;Galectin-1、VEGF与CD105-MVD在宫颈癌中均高表达,提示三者可作为宫颈癌预后的指标。

Objective：To study the expressions of CD105 and Galectin-1,VEGF in cervical cancer and relationship with angiogenesis,invasion and metastasis of cervical cancer.Methods：Immunohistochemistry method was used to detect the expression of Galectins-1 and VEGF,CD105 proteins in 30 cases of normal cervica and 40 cases of cervical cancer,and to analyze their relationship with the development of cervical cancer.Results：The positive rates of Galectin-1 and VEGF in 40 cases of cervical cancer were 70% and 75% respectively,10% and 20% in 30 cases of normal cervical tissue.The expressions of Galectin-1 and VEGF were significantly higher in cervical cancer than in normal cervical tissue（P0.05）.The expression of Galectin-1 and VEGF in cervical cancer were correlated with histological type,FIGO classification and lymph node metastasis（P0.05）,but no relationship with age and general types.CD105 as new blood vessels marker of measured MVD relationship with FIGO classification and lymph node metastasis（P0.05）,but not with patient＇s age and tumor grade.Galectin-1 had positive correlation with VEGF（P=0.043,r=0.321）.There were positive correlations between Galectin-1,VEGF and CD105（P= 0.003,r=0.453）（P=0.016,r=0.379）.Conclusion：The increased expression of Galectin-1 in cervical cancer is closely related to VEGF and involved in invasion and metastasis of cervical cancer.Galectin-1,VEGF and CD105 are overexpressed in cervical cancer,may be as indicators of poor prognosis.