优化传统针刺助产处方对孕晚期大鼠宫颈成熟的影响

Effect of the optimized traditional acupuncture prescription for accouchement on cervical ripening in late-stage pregnant rats

目的:通过对孕晚期大鼠宫颈成熟相关指标的实验研究,从分子生物学角度探讨优化的传统针刺助产处方对宫颈成熟的影响机制。方法:将20只初孕Wistar大鼠随机分为电针组和模型对照组各10只,10只同批次非孕雌鼠作为空白对照组。电针组于妊娠20d时以优化的传统针刺助产处方(先电针"合谷"20min,再加刺"三阴交"5min,选用疏密波2Hz/50Hz)为干预方法,模型对照组及空白对照组不予针刺。3组均采用酶联免疫吸附试验方法对宫颈组织基质金属蛋白酶9(MMP-9)及白介素8(IL-8)进行检测。结果:与空白对照组比较,模型对照组MMP-9及IL-8含量显著升高(均P<0.01);与模型对照组比较,电针组MMP-9及IL-8含量显著升高(P≤0.05)。结论:优化的传统针刺助产处方通过提高晚孕大鼠宫颈组织MMP-9、IL-8的含量,参与并介导宫颈成熟,从分子生物学层面阐明了针刺促宫颈成熟的作用机制。

Objective To observe the mechanism of the optimized traditional acupuncture prescription for accouchement on cervical ripening based on the molecularbiology by observing related indices of cervical ripening in late-stage pregnant rats.Methods Twenty initial pregnant Wistar rats were randomly divided into an electroacupuncture（EA） group（n=10） and a model group（n=10）,and other 10 non-pregnancy female rats with same lot were selected as a blank control group.EA group was treated with the optimized traditional acupuncture prescription for accouchement on the 20th day of pregnant,which performed EA at bilateral ＂Hegu＂（LI 4） for 20 min and then at bilateral ＂Sanyinjiao＂（SP 6） for 5 min with 2 Hz/50 Hz sparse-dense wave,while the other groups without acupuncture intervention.The contents of matrix metalloproteinase 9（MMP-9） and interleukin 8（IL-8） in cervix tissue were detected by ELISA method.Results Compared with the blank control group,the contents of MMP-9 and IL-8 in the model group were increased significantly（both P0.01）.Compared with the model group,the contents of MMP-9 and IL-8 in the EA group were increased significantly（P≤0.05）.Conclusion Optimized traditional acupuncture prescription for accouchement can increase the contents of MMP-9 and IL-8 in cervix tissue of late-stage pregnant rats so as to promote cervical ripening,and the mechanism of EA in promoting cervical ripening is explained from the perspective of molecular biology.