某地区3～6周岁健康儿童干湿化学分析仪6个血清酶项目参考区间的建立与比较研究

Establish and compare the reference intervals of 6 serum enzyme items on the dry and wet chemistry analyzer among healthy children aged from 3 to 6 years in a region

目的建立和比较深圳地区3～6周岁健康儿童干湿化学分析仪6个血清酶项目的参考区间,更好地为临床诊断和治疗服务。方法采用随机分层抽样筛选出391名健康儿童抽取静脉血,用美国强生Vitros 350干式化学分析仪和美国Beck-man Coulter LX20湿化学分析仪进行血清天冬氨酸氨基转移酶(AST)、乳酸脱氢酶(LDH)、丙氨酸氨基转移酶(ALT)、碱性磷酸酶(ALP)、γ-谷氨酰转肽酶(GGT)、肌酸激酶(CK)检测并分析结果。结果该地区3～6周岁健康儿童两种方法内不同性别血清AST、LDH、ALT、ALP、GGT结果比较,差异均无统计学意义(P>0.05);CK结果差异均有统计学意义(P<0.05);6个血清酶项目两种方法间结果比较,差异均有统计学意义(P<0.05)。结论 6个血清酶项目两种方法间结果比较均有显著性差异,应该针对不同方法学分别建立该地区3～6周岁健康儿童干湿化学分析仪的参考区间。

Objective To establish and compare the reference intervals of 6 serum enzyme items on the dry and wet chemistry analyzer among healthy children aged from 3 to 6 years in Shenzhen,provide better references for clinical diagnosis and treatment. Methods The stratified randomized cluster sampling method was used to select 391 healthy children aged from 3 to 6 years,AST. LDH. ALT. ALP.GGT.CK in venous blood were detected by the VITROS 350 and Beckman Coulter LX20, and data were analyzed by statistical methods. Results The reference intervals showed no significant difference between boys and girls except CK on the dry and Wet chemistry analyzer respectively（P〉0.05）. But the results of 6 serum enzyme items showed significant difference between the VITROS 350 and Beckman Coulter LX20（P〈0.05）. Conclusion The results showed significant difference between the VITROS 350 and Beckman Coulter LX20, the reference intervals of 6 serum enzyme items on the dry and wet chemistry analyzer among healthy children aged from 3 to 6 years in Shenzhen should be established according to different methods.