石榴果实酚类物质测定体系优化与不同部位组分及含量测定

Optimization of Detection System for Polyphenol and Its Compositions and Contents in Different Parts of Pomegranate Fruit

以"泰山红"石榴为试材,利用高效液相色谱仪(HPLC)测定成熟期石榴果实中果皮、籽粒和果汁中酚类物质的组分及含量。色谱条件:色谱柱为Kromasil色谱柱(250 mm×4.6 mm,5μm),以乙腈-1%乙酸水溶液为流动相进行梯度洗脱。流速为1.1 ml/min,柱温30℃,检测波长280 nm。结果表明:在石榴皮和石榴籽中检测到13种酚类成分,包括没食子酸、绿原酸、对羟基苯甲酸、表儿茶素、咖啡酸、儿茶素、香草醛、阿魏酸、苯甲酸、根皮苷、槲皮素、肉桂酸、根皮素;在石榴汁中检测到上述12种酚类物质,未检测到表儿茶素。石榴皮中酚类物质的含量最高,其次是石榴汁和石榴籽,其中石榴皮中主要的酸性酚类物质和类黄酮类化合物分别为对羟基苯甲酸(0.828 mg/g)和表儿茶素(0.915 mg/g);石榴汁中分别是对羟基苯甲酸(0.121 mg/g)和儿茶素(0.149 mg/g);石榴籽中分别是咖啡酸(0.026 mg/g)和根皮苷(0.075 mg/g)。

A high performance liquid chromatographic method was developed for the analysis of polyphenol compositions and contents in different parts of ＇ Taishanhong＇ pomegranate fruit including peel, seed and juice. The chromatographic separation was performed on a Kromasil （250mm×4.6mm,5μm））. The mobile phase was acetonitrile and 1% acetate acid solution for gradient elution. The column temperature was 30℃ ; the flow rate was 1.1 ml/min and the wave length was 280 nm. The results indicated that thirteen phenolic compounds were identified in pomegranate peel and seed, ineluding gallic acid, ehlorogenie acid, parahydroxybenzoie acid, epicatechin, caffeic acid, eateehin, vanillin, ferulie acid, benzoic acid, phloridzin, quercetin, cinnamie acid and phloretin. Twelve phenolic compounds were identified in pomegranate juice, and epieatechin was not detected. The content of polyphenols was the highest in pomegranate peel, followed by pomegranate juice and pomegranate seed. The major acidic phenolic compound and flavonoid compound in pomegranate peel were parahydroxybenzoie acid （0. 828 mg/g） and epieatechin （0. 915 mg/g） respectively, while those in pomegranate juice were parahydroxybenzoie acid （0.12 mg/g） and cateehin （0. 149 mg/g） respectively, and those in pomegranate seed were eaffeic acid （0. 026 mg/g） and phloridzin （0. 075 mg/g） respectively.