新荧光试剂双[3-(3,5-二溴吡啶)重氮氨基]-联苯的合成及其荧光分析

Synthesis and fluorescence analysis of bis [3-(3,5-dibromo-pyridine)diazoamino]-biphenyl

合成了标题化合物。其结构经元素分析、红外光谱、核磁共振谱证实。研究表明,在碱性介质中,该试剂于λex/λem=248 nm/304 nm产生荧光,且能与Co(Ⅱ)配合并发生荧光增强,基于此,建立了(BBPDDP)对Co(Ⅱ)的新荧光分析法。该方法的线性范围为6×10-9～9.0×10-7mol/L,检测限为9×10-9mol/L。将其应用于维生素B12注射液的测定,结果满意。

A new fluorescence reagent of bis [ 3-（ 3,5-dibro- rno-pyridine） diazoamino]-biphenyl（BBPDDP） has been synthesized. The structure was confirmed by elemental analysis, I HNMR and IR. A chelate complex was formed by the reaction between BBPDDP and Co（ Ⅱ ） in a weak basic medium, which resulted in fluorescence enhancement. The excitation and emission wave lengths of BBPDDP/Co （ Ⅱ ） system was 248 nm and 304 nm in a basic medium,respectively. It could be used to determine trace Co（ Ⅱ ）.