Sysmex XT-4000i全血细胞分析仪在腹水细胞计数与分类中的应用

Application of automatic hematology analyzer Sysmex XT-4000i in cells counting for ascite fluid

目的评价Sysmex XT-4000i全血细胞分析仪体液模式(RET通道)在腹水细胞计数中的应用价值。方法应用Sysmex XT-4000i全血细胞分析仪(仪器法)和改良牛鲍计数板(手工法)分别计数100份良性病变腹水标本细胞,并进行比较。结果仪器法和手工法进行腹水细胞计数的相关性较好(r>0.900,P=0.000)。2种方法的白细胞、单个核细胞绝对值、多个核细胞绝对值比较,差异无统计学意义[(1.403±5.527)×109/Lvs.(0.858±2.740)×109/L,(0.430±1.172)×109/Lvs.(0.343±0.832)×109/L,(0.969±5.011)×109/Lvs.(0.515±2.371)×109/L;P均>0.05]。仪器法的批内精密度高于手工法;细胞总数、红细胞计数、白细胞计数、单个核细胞计数仪器携带污染率均较低(0.037%、0.000%、0.161%、0.070%),多个核细胞仪器携带污染率为5.633%。结论 Sysmex XT-4000i全血细胞分析仪体液模式可以常规应用于腹水标本计数。

Objective To evaluate the application of body fluid mode in automatic hematology analyzer Sysmex XT-4000i （RET channel） in ascite fluid cells counting. Methods One hundred fresh ascite fluid specimens were used for cell counting by automatic hematology analyzer Sysmex XT-4000i （automatic method） and improved Neubauer counting board （manual method） respectively. The results were compared by statistics. Results A good correlation was found between automatic method and manual method. There was no significant difference of the results in leukocyte[（1.403±5.527）×109/L vs. （0.858±2.740） ×109/L], mononuclear cells[（0.430±1.172） ×109/L vs. （0.343±0.832） ×109/L] and multinucleated cells[（0.969±5.011） ×109/L vs. （0.515±2.371） ×109/L] counting by automatic method and manual method （P 0.05）. The coefficient variation（CV%） of the automatic method was higher than manual method.The carry over rates of total cells, erythrocytes, leukocytes, mononuclear cells were 0.037%, 0.000%, 0.161%, 0.070%, while the carry over rates of multinucleated cells was 5.633%. Conclusion Sysmex XT-4000i can be applied in cells counting for routinely clinical ascite fluid detection.