裸鼠人肝癌原位移植瘤模型的建立

The establishment of models with nude mice orthotopic transplantation tumor in human hepatocellular carcinomas

目的探讨利用人肝癌细胞系,建立裸鼠人肝癌原位移植瘤模型,在肝细胞癌自杀基因治疗的应用价值。方法取人肝癌细胞系Bel7402和HepG2,制备皮下移植瘤,后将肿瘤组织接种于裸鼠肝内,建立裸鼠肝原位移植瘤模型。HE染色进行病理形态学观察。结果裸鼠皮下成瘤率为100.0%(4/4)。肝内接种肿瘤,裸鼠存活率100.0%(32/32),成瘤率81.3%(26/32);镜下观察肿瘤细胞成浸润性生长;肺、肠系膜淋巴结等组织器官未见肿瘤转移灶。结论应用人肝癌细胞系建立的裸鼠肝原位移植瘤模型可以很好地模拟人肝癌的解剖学特征,但肝癌转移模型的建立方法尚需探讨。

Objective To explore the value of models with nude mice orthotopic transplantation tumor in hepatocellular carcinomas suicide gene therapy. Methods Be17402 and HepG2 cell lines were cultured in vivo, then implanted subcutaneously in nude mice, and serially planted the subcutaneous transplantation tumor into liver. The tissues were observed by HE stain. Results The subcutaneous tumor formation rate was 100.0%. Tumors were grown in 26 of 32 mice which were injected subcutaneous transplantation tumor, and all of the mice survived. Tumor cells were observed to be invasive growth, and no tumor metastasis was found in lung, mesenteric lymph nodes and other tissues. Conclusion The establishment of models with nude mice orthotopic transplantation tumor in human hepatocellular carcinoma could imitate well anatomy character of human hepatoeellular carcinoma. The establishment methods still need to be discussed.