知母中甾体皂苷类成分的研究(英文)

Steroidal Saponins from Anemarrhena asphodeloides Bge.

从中药知母(Anemarrhena asphodeloides Bge.)的70%乙醇提取物中分离得到6个化合物,经波谱学方法和与已知样品对照鉴定为:3-O-β-D-glucopyranosyl-(1→2)-β-D-galactopyranosyl-(3β,5β)-pregn-16(17)-ene-20-one(1)、timosaponin AIII(2)、timosaponin BIII(3)、22-hydroxy-5β-furost-3β,15α-diol-3-O-β-D-glucopyranosyl(1→2)-β-D-galactopyranoside(4)、timosaponin G(5)、β-daucosterol(6)。其中化合物1为新天然产物,命名为知母孕甾A,是知母中首次发现的C21甾体类化合物,经HR ESI-MS、1D NMR、2D NMR确定其结构,并首次对其核磁数据进行归属。

A new natural compound, 3-0-β-D-glucopyranosyl- （ 1→2 ） -β-D-galactopyranosyl- （ 3β, 5β ） -pregn-16 （ 17 ） - ene-20-one （timopregnane A, 1）, together with five known stroidal glycosides identified as timosaponin AⅢ （2）, timosaponin BⅢ （3） ,22-hydroxy-5β-furost-3β, 15α-diol-3-0-β-D-glucopyranosyl（1→2） - fl-D-galactopyranoside （4） ,timosaponin G （5） ,β-daucosteml （6） ,were isolated by ODS and silica gel column chromatography from rhizome of Anemarrhena asphodeloides Bge. The structure of compound 1 was elucidated on the basis of extensive 1D and 2D NMR spectroscopic analyses and mass spectrometry. Moreover, the related NMR data of compound 1 were firstly reported.