密穗马先蒿不同萃取部位抗运动性疲劳的体内活性研究

Anti-fatigue Effects of Extracts from Pedicularis Densispica Franch on Exercise Mice

本实验应用小鼠游泳训练疲劳模型,以人参为阳性对照,研究密穗马先蒿不同萃取部位的体内抗运动性疲劳作用。结果实验第四周末密穗马先蒿正丁醇组和正丁醇萃余物组的小鼠疲劳相关症状比单纯运动组明显减轻,体重增加(P<0.01,P<0.05);力竭游泳时间分别比单纯运动组、密穗马先蒿石油醚组和乙酸乙酯组长(P<0.01)。密穗马先蒿正丁醇组和正丁醇萃余物组的小鼠RBC计数、HCT值和HGB浓度分别高于单纯运动组、密穗马先蒿石油醚组和乙酸乙酯组(P<0.01,P<0.05)。因此我们认为密穗马先蒿正丁醇提取物和萃余物具有一定的抗运动性疲劳作用,其机理可能与其改善运动小鼠的贫血状态有关。

This study aimed to investigate anti-fatigue effects of the different extracts from Pedicularis densispica Franch on overtrained mice. The anti-fatigue effects of the different extracts from P. densispica were tested by mice to swim training fatigue model using Ginseng as a positive control. The results showed that mice in the exercise ＋ n-BuOH extracts group and the exercise ＋ remains of n-BuOH extraction group showed significantly lessened symptoms related to fatigue ,increased appetite and body weight compared to the exercise group （P 〈 0.01, P 〈 0.05 ）. The exhaustive swim- ming time of mice in the exercise ＋ n-BuOH extracts group and the exercise ＋ remains of n-BuOH extraction group were observed to be significantly longer than those in the exercise group,the exercise ＋ petroleum ether extracts group and the exercise ＋ EtOAc extracts group after the fourth week （P 〈 0.05,P 〈 0.01 ）. RBC count and HCT values and hemoglobin concentration （HGB） of the exercise ＋ n-BuOH extracts group and the exercise ＋ remains of n-BuOH extraction group were significantly higher than those of the exercise group,the exercise ＋ petroleum ether extracts group and the exercise ＋ EtOAc extracts group （P 〈 0.01 ,P 〈 0.05 ）. This suggested that the n-BuOH extracts and the remains of n-BuOH extraction of P. densispica could significantly delay exercise fatigue symptoms and improve exercise capacity of mice. The mechanism of action might be related to improvement of anemia status.